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  • BG-Maleimide

    Description

    BG-Maleimide is a thiol-reactive building block for the one-step synthesis of SNAP-tag® substrates from thiol-containing precursors such as thiol-modified oligonucleotides. This building block allows you to make custom SNAP-tag substrates for labeling SNAP-tag fusion proteins for a wide range of applications such as the coupling of proteins and solid surfaces such as DNA chips (1). 

    The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

    There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described in this document.

    Figure 1. Structure of BG-Maleimide (MW 489.5 g/mol)

    Quality Control

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Physical Purity (HPLC):
      The purity of the product is determined by HPLC analysis.

    Notes

    1. Dry BG-Maleimide should be stored at -20°C.
    2. The BG-Maleimide building block should be dissolved in anhydrous DMF. Choose a solvent volume that will be compatible with the final use. Unused building block dissolved in DMF should be stored at -20°C.

    References

    1. Jongsma, M.A. and Litjens, R.H. (2006). Proteomics. 6, 2650-2655.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. Cellular Imaging and Analysis FAQs
    1. Reaction Conditions for Chemical Coupling (S9153)
    2. Protocol for Labeling SNAP-tag Fusion Proteins with BG-substrates (S9153)

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