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  • Random Primer 9

    Description

    This mixture of random nonanucleotides is used to prime DNA synthesis in vitro along multiple sites of denatured template DNA (1). This primer-template complex is a suitable substrate for DNA Polymerase I(Klenow Fragment). The newly synthesized complementary DNA is "oligo-labelled" by substituting any radiolabelled nucleotide for the appropriate non-radioactive nucleotide in the reaction mixture. Use of synthetic d(N)9 primer ensures the presence of virtually all sequence combination of nanomer primers which results in equally labelled DNA of high specific activity (1,2)

    Oligolabelling by this method generates probes which can be used to screen gene libraries (3), probe Southern and Northern blots (4,5), and for in situ hybridizations (6).

    Sequence

    5' d(N9) 3' [N = A,C,G,T]

    Properties and Usage

    Storage Temperature

    -20°C

    Notes

    1. 12.4 nmol of S1254 are supplied.

    References

    1. Feinberg, A.P. and Vogelstein, B. (1983). Anal. Biochem.. 132, 6-13.
    2. Feinberg, A.P. and Vogelstein, B. (1984). Anal. Biochem.. 137, 266-267.
    3. Grunstein, M. and Hogness, D.S. (1975). Proc. Natl. Acad. Sci. USA. 72, 3961-3965.
    4. Southern, E.M. (1975). J. Mol. Biol.. 98, 503-517.
    5. Smith, G.E. and Summers, M.D. (1980). Anal. Biochem.. 109, 123-129.
    6. Hasse, A. et al. (1984). Methods Virol.. 7, 189-226.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.