CLIP-tag™ is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. CLIP-tag is a small protein tag based on human O6-alkylguanine-DNA-alkyltransferase (AGT). CLIP-tag substrates are derivatives of benzylcytosine (BC). In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag forming a stable thioether link. Although CLIP-tag is based on the same protein as SNAP-tag®, the benzylcytosine substrates form a separate class of substrates, different from the benzylguanine substrates recognized by SNAP-tag. CLIP-tag and SNAP-tag can be used for orthogonal simultaneous labeling.
CLIP-tag Purified Protein can be used as a positive control for in vitro labeling with various CLIP-tag fluorescent substrates. The coding sequence of CLIP-tag was cloned into a pTXB1 derived E. coli T7 expression vector. CLIP-tag protein (MW: 20,675) was expressed and purified according to the instructions in the IMPACT™ kit manual (NEB #E6901 ). The purified CLIP-tag protein was dialyzed into 1X phosphate buffered saline (PBS) solution containing 1 mM DTT at 1 mg/ml (50 μM) and stored at -80°C.
Mass spectrometry analysis indicates the CLIP-tag purified protein has two species with molecular weights of 20,675 and 20,810 (with a C-terminal DTT moiety attached).
Legal and Disclaimers
Research Use Only
- Research Use Only. No other use is authorized, including without limitation Commercial Use. Commercial Use means any and all uses of the product and derivatives by a party for monetary or other consideration including, but not limited to:
(1) product manufacture;
(2) providing of a service, information or data;
(3) resale of the product, derivatives or components;
(4) use of the product, derivatives or components by therapeutic, diagnostic or prophylactic clinical or commercial purposes.
Information regarding any other license may be obtained by contacting Business Development and Licensing, New England Biolabs, Inc., 240 County Road, Ipswich, MA 01938. Tel: 978-927-5054, Fax: 978-921-1350.
Notice To Buyer/User: The Buyer/User has a non-exclusive license to use this system or any component thereof for RESEARCH AND DEVELOPMENT PURPOSES ONLY. Commercial use of this system or any components thereof requires a license from New England Biolabs, Inc., 240 County Road, Ipswich, MA 01938.
Cellular Imaging and Analysis (i.e., SNAP and CLIP products)
Notice to Buyer/User: The Buyer/User has a non-exclusive license to use this system or any component thereof for RESEARCH AND DEVELOPMENT ONLY. Commercial use of this system or any components thereof requires a license from New England Biolabs, Inc., 240 County Road, Ipswich, MA 01938.
The products and/or their use may be covered by one or more of the following patents and patent applications:
7,939,284 (Methods for Using O6-Alkylguanine-DNA-Alkyltransferases)
7,888,090 (Mutants of O6-Alkylguanine-DNA-Alkyltransferases)
8,163,479 Specific Substrates for O6-Alkylguanine-DNA-Alkyltransferases)
8,178,314 (Pyrimidines Reacting With O6-Alkylguanine-DNA-Alkyltransferases)
PCT/EP2007/057597 (Labeling of Fusion Proteins with Synthetic Probes)
EP07117800 (Drug Delivery)
EP07117802 (Drug Delivery)
EP07120288 (GTPase-Transient Protein Protein Interactions)
These patents and patent applications are owned by Covalys, or owned by the Ecole Polytechnique Fédérale de Lausanne (EPFL) and exclusively licensed to Covalys and NEB.