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  • Proteinase K

    This product has been discontinued and replaced by a new Proteinase K (P8107).

    Discontinued Date

    08/30/2013
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    Categories:
    Discontinued Products

    Description

    Proteinase K is a non-specific, subtilisin-related serine protease with a very high specific activity. Proteinase K is not inactivated by chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH (4-12.5).

    Product Source

    Tritirachium album

    Properties and Usage

    Unit Definition

    One unit is defined as the amount of enzyme required to liberate folin-positive amino acids and peptides corresponding to 1 µmol of tyrosine in 1 minute at 37°C in a total reaction volume of 250 µl (1).

    Usage Concentration

    20 mg/ml

    Storage Temperature

    -20°C

    Storage Conditions

    20 mM Tris-HCl
    1 mM CaCl2
    50% Glycerol
    pH 7.4 @ 25°C

    Specific Activity

    30 units/mg

    Quality Control

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Endonuclease Activity (Nicking):
      The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
    • Exonuclease Activity (Radioactivity Release):
      The product is tested in a reaction containing a radiolabeled mixture of single and double-stranded DNA. After incubation for 4 hours the exonuclease activity is determined by the % release of radioactive nucleotides.
    • Non-Specific DNase Activity (16 hour):
      The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.
    • RNase Activity (2 Hour Digestion):
      The product is tested in a reaction containing a RNA substrate.  After incubation for 2 hours there is no detectable degradation of the RNA substrate as determined by gel electrophoresis.

    References

    1. Anson, M. L. (1939). J. Gen. Physiol.. 22, 79.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. What is the Proteinase K activity in commonly used buffers?
    2. Why was the previous Proteinase K product (P8102) discontinued?

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