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  • Prestained Protein Marker, Broad Range (7-175 kDa)

    Description

    Prestained Protein Marker, Broad Range is a mixture of purified proteins covalently coupled to a blue dye that resolves to 8 bands when electrophoresed. The protein concentrations are carefully balanced for even intensity. The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDS-PAGE gels relative to unstained proteins (1). The apparent molecular weight of the prestained proteins are given in the table below. For precise molecular weight determinations, use NEB's unstained Protein Marker, Broad Range (NEB #P7702) or Protein Ladder (NEB #P7703) in addition to the prestained marker.

    P7708_thumb

    10-20% SDS PAGE. This marker was recently recalibrated using the Protein Ladder (NEB #P7703), and the revised apparent molecular weights are shown. The formulation of the marker is unchanged.

    Properties and Usage

    Storage Temperature

    -20°C

    Storage Conditions

    70 mM Tris-HCl
    33 mM NaCl
    40 mM DTT
    1 mM EDTA
    10% Glycerol
    0.01% Phenol red
    2% SDS
    pH 6.8 @ 25°C

    Notes

    1. Recommended load for SDS-PAGE is 15 μl, while 6 μl is recommended for Western blot analysis.
    2. Apparent molecular weights of every lot are determined on Invitrogen Novex 10-20% Tris-glycine SDS PAGE gels using NEB's Protein Ladder (NEB #P7703).
    3. Approximate protein concentration 0.1 mg/ml to 0.2 mg/ml. 

    References

    1. Laemmli, U.K. (1970). Nature. 227, 680.
    2. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, (2nd ed.). Cold Spring Harbor Laboratory Press.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. What controls can be done for a Western blot?
    2. Why do the apparent molecular weight values for the prestained protein markers appear to be different in different gel types?
    1. Loading a Sample (P7708)
    2. Using NEB Protein Markers & Ladders
    Prestained proteins carry a charge because of the dye molecules bound to the protein.
    Because of this, any changes in the running conditions will alter their migration. 
    These products should not be used for accurate molecular weight determination. 
    The unstained markers and ladders are better for that. 
    Prestained are best designed for wesDescriptiontern blot applications.