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  • TriDye™ 1 kb DNA Ladder

    Description

    TriDye™ 1 kb DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis.

    The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 0.5-10.0 kilobases. The 3.0 kb fragment has increased intensity to serve as a reference band.

    TriDye During Electrophoresis: On a standard 1% agarose gel in 1X TBE, xylene cyanol FF migrates at approximately 4 kb, bromophenol blue at approximately 300 bp and the orange G at approximately 50 bp. As the percentage of agarose changes, the migration rates of the dyes relative to migration rates of the DNA will change.


    N3272Fig2_thumb

    1 kb DNA Ladder visualized by ethidium bromide staining on a 0.8% TAE agarose gel. Mass values are for 0.5 µg/lane.

    Properties and Usage

    Bases

    FragmentMassbp
    14210002
    2428001
    3506001
    4425001
    5334001
    61253001
    7482000
    8361500
    9421000
    10a21517
    10b21500

    Effective Size Range

    500bp to 10,002bp

    Storage Temperature

    4°C

    Storage Conditions

    10 mM Tris-HCl
    10 mM EDTA
    10% Glycerol
    0.006% bromophenol blue
    0.06% Orange G
    0.006% Xylene Cyanol
    pH 7.9 @ 25°C

    Notes

    1. We recommend loading 10 μl (0.5 μg) of TriDye DNA Ladder per gel lane.
    2. In general, it is recommended to load 1ul per mm of gel lane.
    3. The TriDye DNA Ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
    4. TriDye DNA Ladder is stable for at least 6 months at 25°C.
    5. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.

    References

    1. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual (2nd Ed.). 10.51-10.67.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
    2. How can I quantify the amount of DNA in each band of a marker?
    3. Can I use GelRed with the DNA Ladders from NEB?
    4. Can I use SYBR® with the DNA Ladders from NEB?
    5. Can I use Midori Green with the DNA Ladders from NEB?

    Selection Tools

    Mix well before use, especially after thawing.