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  • Mass DNA Ladder

    Description

    The Mass DNA ladder is a pre-mixed, ready-to-load molecular weight marker containing Orange G dye as a tracking dye. 

    The DNA Ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 7 bands suitable for size determination and mass quantification of linear double-stranded DNA fragments. The digested DNA includes fragments ranging from 0.5 to 10 kilobases.

    Comes supplied with 1 vial of Gel Loading Dye, Orange (6X).



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    Mass DNA Ladder. Mass values are for .75 μg/lane

    Properties and Usage

    Bases

    FragmentMassbp
    124010002
    21806001
    31204001
    4753001
    5602017
    6451008
    730500

    Preparation

    The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.

    Effective Size Range

    500bp to 10,002bp

    Storage Temperature

    4°C

    Storage Conditions

    3.3 mM Tris-HCl
    11 mM EDTA
    0.15% Orange G
    0.017% SDS
    2.5% Ficoll®-400
    pH 8.0 @ 25°C

    Notes

    1. The 500bp band comprises 4 DNA fragments ranging in size between 494 and 517bp.

    2. Preparation: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.

    3. Usage Recommendation: We recommend loading 5 to 20 µl (0.25 µg to 1 µg) of the Mass DNA Ladder per gel lane. For a more accurate mass estimation, always compare the samples intensity to the ladder’s band of nearest size. The loading volume and the loading buffer for the samples and the Mass DNA Ladder should be the same whenever possible.

    4. Mass DNA Ladder is stable for at least 6 months at 25°C.

    5. For long term storage. Store at 4°C or -20°C. If stored at -20°C, mix well after thawing.

    References

    1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Cold Spring Harbor(Ed.), 10.51-10.67.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. How much Mass DNA Ladder should I load on a gel?
    2. Can I store it at -20°C?
    3. Can I use GelRed with the DNA Ladders from NEB?
    4. Can I use SYBR® with the DNA Ladders from NEB?

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