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  • PCR Marker

    Description

    The PCR Marker consists of a proprietary plasmid that is digested to completion with appropriate restriction enzymes to yield 5 double-stranded DNA bands suitable for use as molecular weight standards for agarose and acrylamide gel electrophoresis. The digested DNA includes fragments ranging from 50-766 base pairs. The approximate mass of DNA in each of the bands is provided (assuming a 0.3 μg load) for approximating the mass of DNA in comparably intense samples of similar size.

    Comes supplied with 1 vial of Gel Loading Dye, Blue (6X).



    N3234_thumb

    PCR Marker visualized by ethidium bromide staining on a 1.8% TBE Agarose Gel. Mass values are for 0.3 μg/lane.

    Properties and Usage

    Bases

    FragmentMassbp
    162766
    240500
    348300
    461150
    58950

    Effective Size Range

    50bp to 766bp

    Storage Temperature

    4°C

    Storage Conditions

    10 mM Tris-HCl
    1 mM EDTA
    pH 8.0 @ 25°C

    Notes

    1. We recommend loading 0.3 μg of PCR Marker diluted in sample buffer.
      This marker was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
    2. PCR Marker is stable for at least 3 months at 4°C. 
    3. For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O.
    4. All ends have 5' overhangs that can be labeled using T4 Polynucleotide Kinase (NEB #M0201) or filled-in using DNA Polymerase I, Klenow Fragment (NEB #M0210) (1). Use α-[32P] dCTP or α-[32P] dGTP for the fill-in reaction.
    5. 1X Gel Loading Dye, Blue:
      2.5% Ficoll-400
      11 mM EDTA
      3.3 mM Tris-HCL (pH 8.0@ 25°C) 
      0.017% SDS
      0.015% bromophenol blue

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
    2. How can I quantify the amount of DNA in each band of a marker?
    3. Can I use GelRed with the DNA Ladders from NEB?
    4. Can I use Midori Green with the DNA Ladders from NEB?
    1. End-labeling Protocol
    2. Suggested protocol for loading a sample (PCR Marker)

    Selection Tools

    To make it ready-to-load, dilute in TE buffer instead of water.