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  • 100 bp DNA Ladder

    Did you know this product can be customized or purchased in larger volumes? Submit an inquiry to find out more.

    Now comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS (B7025).
    Catalog #SizeConcentrationGel LanesPriceQtyAdd to Cart
    N3231S0.1 ml500 μg/ml100$54.00Add to Cart
    N3231L0.5 ml500 μg/ml500$216.00Add to Cart
      
    Categories:
    DNA Markers & Ladders Products
    Applications:
    DNA Analysis

    Description

    A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100-1,517 base pairs. The 500 and 1,000 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each of the bands is provided (assuming a 0.5 μg load) for approximating the mass of DNA in comparably intense samples of similar size.

    Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS



    N3231_thumb

    100 bp DNA Ladder visualized by ethidium bromide staining on a 1.3% TAE agarose gel. Mass values are for 0.5 µg/lane.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    Gel Loading Dye, Purple (6X), no SDS256X

    Properties and Usage

    Bases

    FragmentMassbp
    1451517
    2351200
    3951000
    427900
    524800
    621700
    718600
    897500/517
    938400
    1029300
    1125200
    1248100

    Effective Size Range

    100bp to 1,517bp

    Storage Temperature

    -20°C

    Storage Conditions

    10 mM Tris-HCl
    1 mM EDTA
    pH 8.0 @ 25°C

    Notes

    1. This ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
    2. We recommend loading 0.5 μg of 100 bp DNA Ladder diluted in sample buffer.
    3. All fragments have 4-base, 5´ overhangs that can be end labeled using T4 Polynucleotide Kinase (#M0201) or filled-in using DNA Polymerase I, Klenow Fragment (#M0210) (1). Use α-[32P] dATP or α-[32P] dTTP for the fill-in reaction.
    4. 100 bp DNA Ladder is stable for at least 3 months at 4°C.
    5. For long term storage store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O.
    6. Due to the limitations of the acrylamide gel technology, one or two extra bands may be visible on the DNA ladders when run on a polyacrylamide gel.
    7. 1X Gel Loading Dye, Purple (6X), no SDS:
      2.5% Ficoll®-400 
      10mM EDTA 
      3.3mM Tris-HCl (pH 8.0@25°C)
      0.02% Dye 1 
      0.001% Dye 2 

    References

    1. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual (2nd Ed.). 10.51-10.67.

    FAQs

    1. Why are there extra bands visible on polyacrylamide gels?
    2. Why is the separation of the lower bands incomplete?
    3. What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
    4. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
    5. How can I quantify the amount of DNA in each band of a marker?
    6. Can I use GelRed with the DNA Ladders from NEB?
    7. Can I use Midori Green with the DNA Ladders from NEB?
    8. Can I use SYBR® with the DNA Ladders from NEB?

    Tech Tips

    To make it ready-to-load, dilute in TE buffer instead of water.

    Protocols

    1. End-labeling Protocol
    2. Suggested protocol for loading a DNA Ladder/marker

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Selection Tools

    Certificate of Analysis

    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    Specifications

    The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.