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  • pNEB193 Vector

    Discontinued Date

    01/01/2013
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    Categories:
    Discontinued Products

    Description

    pNEB193 is a pUC19 derivative that carries unique AscI, PmeI, and PacI sites. The only differences between pUC19 and pNEB193 are in the polylinker region. A unique AscI site (GGCGCGCC) is located between the BamHI site and the SmaI site. A unique PacI site (TTAATTAA) is located between the BamHI site and the XbaI site, and a unique PmeI site (GTTTAAAC), is located between the PstI site and the SalI site.

    The polylinker does not interrupt the lacZ reading frame. All or part of the polylinker can be easily excised with the appropriate restriction endonucleases and placed in your own DNA vector.

    Product Source

    pNEB193 is isolated from E. coli TB1(hsd M+) by a standard plasmid purification procedure.

    Properties and Usage

    Copy Number

    High (100+)

    Origin of Replication

    • pMB1 (high-copy mutant)

    Storage Temperature

    -20°C

    Storage Conditions

    10 mM Tris-HCl
    1 mM EDTA
    pH 8.0 @ 25°C

    Notes

    1. The AscI site is also a unique BssHII site in pNEB193.
    2. E. coli strain TB1 is recommended for transformations.

    References

    1. Guan, C. Unpublished observation

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. How homogeneous is the DNA/ how much is supercoiled?
    2. Are the plasmids cesium purified?
    3. What primers should I use to sequence an insert (pUC19, pNEB193, LITMUS)?

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