• My NEB
  • Print
  • PDF
  • pUC19 Vector

    Description

    pUC19 is a commonly used plasmid cloning vector in E.coli. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1).
    IMAGE WITH CAPTION
    Modify text and image as needed. In order for the modal to work, must update anchor href to point to updated image.

    DNASU is a central repository for plasmid clones and collections that may also be helpful.

    Product Source

    pUC19 is isolated from E. coli ER2272 (dam+ dcm+ EcoK M-) by a standard plasmid purification procedure.

    Polylinker DNA Sequence

    GAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTT 

    GENBANK Assession Number

    L09137

    Properties and Usage

    General Utility

    Cloning

    Copy Number

    High (100+)

    Origin of Replication

    • pMB1 (high-copy mutant)

    Storage Temperature

    -20°C

    Storage Conditions

    10 mM Tris-HCl
    1 mM EDTA
    pH 8.0 @ 25°C

    References

    1. Yanisch-Perron, C., Vieira, J. and Messing, J. (1985). Gene. 33, 103-119.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. How homogeneous is the DNA/ how much is supercoiled?
    2. Are the plasmids cesium purified?
    3. What primers should I use to sequence an insert (pUC19, pNEB193, LITMUS)?

    Interactive Tools