pBR322 Vector

Description

pBR322 DNA is a commonly used plasmid cloning vector in E. coli (1). The molecule is a double-stranded circle 4,361* base pairs in length (2). pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol. The molecular weight is 2.83 x 106 daltons.

*Sequencing data from Watson (confirmed at New England Biolabs, Inc.) has shown pBR322 to be 4,361 base pairs, not 4,363 base pairs as previously reported.

DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.

Product Source

pBR322 is isolated from E. coli ER2686 (dam +dcm + EcoK M-) by a standard plasmid purification procedure.



pBR322_map

Properties and Usage

Copy Number

Medium (10-100)

Origin of Replication

  • pMB1

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C

References

  1. Bolivar, F., Rodriguez, R.L., Greene, P.J., Betlach, M.C., Heynecker, H.L. and Boyer, H.W. (1977). Gene. 2, 95-113.
  2. Watson, N. (1988). Gene. 70, 399-403.

FAQs

  1. How homogeneous is the DNA/ how much is supercoiled?
  2. Are the plasmids cesium purified?

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Interactive Tools

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.