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  • pBR322 DNA-MspI Digest

    Description

    The MspI digest of pBR322 DNA yields 26 fragments. Suitable for use as molecular weight standards for agarose gel electrophoresis (1,2).

    Comes supplied with 1 vial of Gel Loading Dye, Blue (6X).



    N3032_thumb

    pBR322 DNA-MspI Digest visualized by ethidium bromide staining. 1.8% agarose gel.

    Properties and Usage

    Bases

    FragmentMassbp
    1143622
    2121527
    393404
    470307
    555242
    655238
    750217
    846201
    944190
    1041180
    11, 1274160
    13, 1468147
    1528123
    1625110
    172190
    181776
    191567
    20, 211634
    22, 231226
    24315
    25, 2649

    Effective Size Range

    9bp to 622bp

    Storage Temperature

    -20°C

    Storage Conditions

    10 mM Tris-HCl
    1 mM EDTA
    pH 8.0 @ 25°C

    Notes

    1. Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O.

      1X Gel Loading Dye, Blue:
      2.5% Ficoll-400
      11 mM EDTA
      3.3 mM Tris-HCl (pH 8.0@25°C)
      0.017% SDS
      0.015% bromophenol blue

    References

    1. Sutcliffe, J.G. (1978). Cold Spring Harbor Symp. Quant. Bio.. 43, 77-90.
    2. Peden, K.W.C. (1983). Gene. 22, 277-280.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. Why is the separation of the lower bands incomplete?
    2. What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
    3. How can I quantify the amount of DNA in each band of a marker?
    4. Can I use GelRed with the DNA Ladders from NEB?
    5. Can I use SYBR® with the DNA Ladders from NEB?
    6. Can I use Midori Green with the DNA Ladders from NEB?
    1. Suggested protocol for loading a sample (N3032)
    2. Suggested protocol for loading a sample
    To make it ready-to-load, dilute in TE buffer instead of water.