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  • pBR322 DNA-BstNI Digest

    Description

    The BstNI Digest of pBR322 DNA yields 6 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1,2).

    Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.



    N3031_thumb

    pBR322 DNA-BstNI Digest visualized by ethidium bromide staining. 1.4% agarose gel.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    Gel Loading Dye, Purple (6X), no SDS256X

    Properties and Usage

    Bases

    FragmentMassbp
    14261,857
    22431,058
    3213929
    488383
    528121
    6313

    Effective Size Range

    13bp to 1,857bp

    Storage Temperature

    -20°C

    Storage Conditions

    10 mM Tris-HCl
    1 mM EDTA
    pH 8.0 @ 25°C

    Notes

    1. For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O.

      1X Gel Loading Dye, Purple, no SDS: 
      2.5% Ficoll®-400
      10mM EDTA
      3.3mM Tris-HCl (pH 8.0@25°C)
      0.02% Dye 1
      0.001% Dye 2

    References

    1. Sutcliffe, J.G. (1978). Cold Spring Harbor Symp. Quant. Bio.. 43, 77-90.
    2. Peden, K.W.C. (1983). Gene. 22, 277-280.

    FAQs

    1. Why is the separation of the lower bands incomplete?
    2. What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
    3. How can I quantify the amount of DNA in each band of a marker?
    4. Can I use GelRed with the DNA Ladders from NEB?
    5. Can I use Midori Green with the DNA Ladders from NEB?
    6. Can I use SYBR® with the DNA Ladders from NEB?

    Tech Tips

    To make it ready-to-load, dilute in TE buffer instead of water.

    Protocols

    1. Suggested protocol for loading a DNA Ladder/marker

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Certificate of Analysis

    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    Material Safety Data Sheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.