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  • λ DNA-Mono Cut Mix

    Description

    The Mono Cut Mix is a mixture of intact lambda DNA and lambda DNA separately digested with ApaI, KpnI, XbaI and XhoI. The fragments have been filled in with DNA Polymerase I Large (Klenow) Fragment to prevent re-annealing. These fragments are best separated by Pulsed Field Gel Electrophoresis.The approximate mass of DNA in each of the bands is provided (assuming a 0.5 μg load) for approximating the mass of DNA in comparably intense samples of similar size.

    Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.



    N3019_thumb

    PFGE separation of 0.5 µg of Lambda Mono Cut Mix. 1% agarose gel, 0.5X TBE, 6 V/cm, 15°C for 20 hours. Switch times ramped from 0.5-1.5 seconds.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    Gel Loading Dye, Purple (6X), no SDS256X

    Properties and Usage

    Bases

    FragmentMassbp
    110048502
    27938416
    36933498
    46229946
    55124508
    64923994
    73517053
    83115004
    92110086
    1031503

    Effective Size Range

    1,503bp to 48,502bp

    Storage Temperature

    -20°C

    Storage Conditions

    10 mM Tris-HCl
    1 mM EDTA
    pH 8.0 @ 25°C

    Notes

    1. These fragments are best separated by Pulsed Field Gel Electrophoresis. Alternatively Lambda Mono Cut Mix can be separated by conventional gel electrophoresis using the following conditions: 0.5 μg Lambda Mono Cut Mix, 0.4 % agarose gel, 1X TAE, 20-30 V, 25°C for 24 hours.
    2. 1X Gel Loading Dye, Purple, no SDS: 
      2.5% Ficoll®-400
      10mM EDTA
      3.3mM Tris-HCl (pH 8.0@25°C)
      0.02% Dye 1
      0.001% Dye 2

    References

    1. Daniels, D.L. et al. (1983). Appendix II: Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R.A. Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.

    FAQs

    1. How can I quantify the amount of DNA in each band of a marker?
    2. General information on Lambda DNA Mono Cut Mix:
    3. Can I use GelRed with the DNA Ladders from NEB?
    4. Can I use Midori Green with the DNA Ladders from NEB?
    5. Can I use SYBR® with the DNA Ladders from NEB?

    Tech Tips

    To make it ready-to-load, dilute in TE buffer instead of water.

    Protocols

    1. Suggested protocol for loading a DNA Ladder/marker

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Certificate of Analysis

    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.