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  • DNA Gyrase (E. coli) Substrate


    A double-stranded covalently closed circular DNA molecule produced by incubating supercoiled pUC19 DNA with Topoisomerase I (E. coli).

    Product Source

    pUC19 is isolated from E. coli ER2272 (dam+ dcm+ EcoK M– ) by a standard plasmid purification procedure

    Properties and Usage

    Storage Temperature


    Storage Conditions

    10 mM Tris-HCl
    1 mM EDTA
    pH 8.0 @ -20°C


    1. Yanisch-Perron, C., Vieira, J. and Messing, J. (1985). Gene. 33, 003-119.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.


    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Usage Guidelines & Tips