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  • M13KE Phage

    Description

    M13KE Phage is a suspension of infectious virions derived from the Ph.D. cloning vector, M13KE (1). The vector phage may be a useful control for phage ELISA or for titering of phage stocks. For cloning, the isolated replicative form of M13KE is available as part of the Ph.D. Peptide Display Cloning System (NEB #E8101S).

    Properties and Usage

    Affinity Tag

    Other

    Storage Temperature

    -20°C

    Quality Control

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Absolute Phage Titer:
      Infection of a mid-log culture of E. coli with phage followed by plating, yields ≥ the specified pfu/ml.
    • Cloning Site Verification:
      From infected E. coli, dsDNA is purified. The DNA was sequenced across the MCS and digested with KpnI and EagI to confirm the presence of restriction enzyme cloning sites.
    • Wild-type M13 contamination:
      The product is titered on plates containing X-gal and IPTG to ensure there is no contamination of wild-type M13 phage. Blue plaques are observed at least 500:1 over clear plaques.

    References

    1. Noren, K. A. and Noren, C. J. (2001). Methods. 23, 169-178.
    2. Sambrook, J. and Russell, D. W. (2001). Molecular Cloning: A Laboratory Manual. 3rd ed., 3.17-3.32.

    Supporting Documents

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. What is Ph.D.™ Phage Display?
    2. What peptide libraries are available for use with Ph.D.™ Phage Display?
    1. M13 Amplification

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    Application Notes