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  • Mung Bean Nuclease

    Description

    A single-strand specific DNA and RNA endonuclease which will degrade single-stranded extensions from the ends of DNA and RNA molecules, leaving blunt, ligatable ends.

    Highlights

    • Removal of single-stranded extensions (3' and 5') to leave ligatable blunt ends
    • Transcriptional mapping
    • Cleavage of hairpin loops
    • Supplied with 10X Reaction Buffer

    Product Source

    Mung bean sprouts.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    Mung Bean Nuclease Reaction Buffer10X

    Advantages and Features

    Applications

    • Removal of 3´ and 5´ extensions or single-stranded regions from DNA or RNA termini
    • Transcriptional mapping
    • Cleavage of hairpin loops
    • Excision of gene coding sequences from genomic DNA
    • Generation of new restriction sites

    Properties and Usage

    Unit Definition

    One unit is defined as the amount of enzyme required to produce 1 µg of acid-soluble total nucleotide in a total reaction volume of 50 μl in 1 minute at 37°C in 1X Mung Bean Nuclease Reaction Buffer with 0.5 mg/ml denatured Calf Thymus DNA.

    Reaction Conditions

    1X Mung Bean Nuclease Reaction Buffer
    Incubate at 30°C

    1X Mung Bean Nuclease Reaction Buffer:
    30 mM NaCl
    50 mM sodium acetate
    1 mM ZnSO4
    pH 5 @ 25°C

    Storage Conditions

    10 mM sodium acetate
    0.1 mM Zinc Acetate
    1 mM Cysteine
    50% Glycerol
    0.001% Triton® X-100
    pH 5.0 @ 25°C

    Molecular Weight

    Theoretical: 39 kDa

    Quality Control

    Quality Assurance Statement

    • Purified free of double-strand exonuclease contamination.

    Notes

    1. It is no longer necessary to supplement Mung Bean Nuclease reactions with Zn2+. The zinc acetate in the storage buffer fulfills the Zn2+ requirement of the enzyme even after dilution in a reaction.

    References

    1. Kowalski, D. et al. (1976). Biochemistry. 15, 4457-4463.
    2. McCutchan, T.F. et al. (1984). Science. 225, 626-628.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. Should I use Mung Bean Nuclease or DNA Polymerase I, Large (Klenow) Fragment (NEB# M0210)?
    2. Is the Mung Bean Nuclease active in other NEBuffers?
    3. Can Mung Bean Nuclease be heat inactivated?
    4. Does Zn2+ need to be added to a Mung Bean Nuclease reaction?
    5. Will Mung Bean Nuclease degrade double stranded DNA?
    1. Removal of Single-Stranded Extension

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    Usage Guidelines & Tips