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Catalyzes the stepwise removal of mononucleotides from 3´-hydroxyl termini of duplex DNA (1). A limited number of nucleotides are removed during each binding event, resulting in coordinated progressive deletions within the population of DNA molecules (2). Exonuclease III has also been reported to have RNase H, 3´-phosphatase and AP-endonuclease activities (1).
The preferred substrates are blunt or recessed 3´-termini, although the enzyme also acts at nicks in duplex DNA to produce single-strand gaps. 3´-protruding termini are resistant to cleavage; the degree of resistance depends on the length of the extension,with extensions 4 bases or longer being essentially resistant to cleavage.
Exonuclease III activity depends partially on helical structure (4) and displays sequence dependence (C>A=T>G; ref. 5). Temperature, salt concentration and the ratio of enzyme to DNA greatly affect enzyme activity, requiring reaction conditions to be tailored to specific applications.Product Source
An E. coli strain that carries the cloned Exonuclease III gene from E. coli.- This product is related to the following categories:
- Exonucleases and Non-specific Endonucleases Products
- This product can be used in the following applications:
- Polymerases for DNA Manipulation
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