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  • IsoAmp® II Universal tHDA Kit

    Description

    Thermophilic Helicase-Dependent Amplification (tHDA) is a novel method for isothermal amplification of nucleic acids. Like PCR, the tHDA reaction selectively amplifies a target sequence defined by two primers. However, unlike PCR, tHDA uses an enzyme called a helicase to separate DNA, rather than heat. This allows DNA amplification without the need for thermocycling. The tHDA reaction can also be coupled with reverse transcription for RNA analysis.

    IsoAmp® II Universal tHDA kit can be used to amplify and detect short DNA sequences (70 bp - 120 bp) at a constant temperature. The kit can be used with a variety of templates, including microbial genomic DNA, viral DNA, plasmid DNA, and cDNA. A single copy of target DNA can be amplified by tHDA and detected by agarose gel electrophoresis when optimized primers and buffer are used.

     IsoAmp® II Universal tHDA kit is based on a second-generation thermophilic Helicase-Dependent Amplification platform. The reactions supported by IsoAmp® II Universal tHDA kit include tHDA, reverse transcription HDA (RT-HDA), real-time quantitative HDA (qHDA) and real-time quantitative RT-HDA (qRT-HDA), from a single reaction buffer. IsoAmp® II Universal tHDA kit contains an enzyme mix, dNTP solution and buffer, allowing flexibility in reaction setup. A Control Template and a set of specific primers are supplied for the positive control reaction.

    Reommended storage conditions for the tHDA kit are -20°C for storage shorter than 6 months and -80°C for storage greater than 6 months. Please avoid repeated freeze-thaw cycles as this may decrease performance of the kit.







    Figure 1.
    HDA Technology.
    Figure 2.
    Positive control assays with varying amount of Control Template. A, two-step tHDA positive control with 10 pg - 1 ng Control Template. B, two-step qHDA positive control using 500 fg - 5 ng, in triplicate.

    Properties and Usage

    Storage Temperature

    -20°C

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.
    1. How to design the primer and select the amplicon for IsoAmp II Universal tHDA Kit?
    1. One-Step tHDA (thermostable HDA)
    2. One-Step RT-HDA (Reverse Transcription tHDA)
    3. One-Step qHDA (Real-time quantitative tHDA)
    4. One-Step qRT-HDA (Real-time quantitative RT-HDA)
    5. Two-Step tHDA (thermostable HDA)
    6. Two-Step RT-HDA (Reverse Transcription tHDA)
    7. Two-Step qHDA (Real-time quantitative tHDA)
    8. Two-Step qRT-HDA (Real-time quantitative RT-HDA)