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  • Anti-MBP Antiserum

    This product has been discontinued. Anti-MBP Monoclonal Antibody (E8032S/L) is a suitable alternative for most applications.
    • This product was discontinued on 09/11/2013
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    Discontinued Products

    Description

    Rabbit antiserum prepared by immunizing against affinity-purified maltose-binding protein.

    Properties and Usage

    Recommended Dilution for Western Blot

    1/10,000

    Storage Temperature

    -20°C

    Notes

    1. Store at -20°C undiluted. May be stored at 4°C diluted in buffer containing 1 mM NaN3.

    Protocols

    1. Western Transfer Protocol for Anti-MBP Antiserum

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Quality Control

    Quality Assurance Statement

    • In an ELISA assay, a dilution of 1/10,000 added to a microtiter plate containing 1 µg purified maltose-binding protein per well gives a value of approximately 1.0 at OD490 after 8 minutes reaction time, using OPD as the substrate for horseradish peroxidase. The same dilution gives a strong signal when used to detect maltose-binding protein in Western blots developed with a variety of detection systems. Once diluted, the serum may be reused a few times in Western blots.

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.