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  • NEBNext® mRNA Library Prep Master Mix Set for Illumina®


    The NEBNext® mRNA Library Prep Master Mix Set for Illumina® contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. Each of these components must pass rigorous quality control standards and are Lot Controlled, both individually and as a set of reagents.

    Functional Validation
    Each set of reagents is functionally validated together through construction and sequencing of an RNA library on the Illumina sequencing platform.

    Lot Control

    The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    NEBNext RNA Fragmentation Buffer10X
    NEBNext RNA Fragmentation Stop Solution10X
    Linear Acrylamide10 mg/ml
    Random Primers
    Murine RNase Inhibitor
    NEBNext First Strand Synthesis Reaction Buffer5X
    NEBNext Second Strand Synthesis Enzyme Mix
    NEBNext Second Strand Synthesis Reaction Buffer10X
    NEBNext End Repair Enzyme Mix
    NEBNext End Repair Reaction Buffer10X
    NEBNext dA- Tailing Reaction Buffer10X
    Quick T4 DNA Ligase
    NEBNext Quick Ligation Reaction Buffer5X
    Nuclease-free Water
    Phusion High-Fidelity PCR Master Mix with HF Buffer2X
    NEBNext High-Fidelity 2X PCR Master Mix (SAMPLE)2X
    ProtoScript® II Reverse Transcriptase200,000 units/ml
    Klenow Fragment (3´→ 5´ exo)

    Properties and Usage

    Materials Required but not Supplied

    • 3 M Sodium Acetate, pH 5.2
    • 100% Ethanol
    • 70% Ethanol
    • PCR Column Purification Kit (Qiagen or other)
    • DNA Gel Extraction Column Purification Kit
    • DNA Adaptors and Primers or Vector
    • Size Selection Materials [E-Gel® (Life Technologies, Inc.), Agarose Gel or AMPure® (Beckman Coulter, Inc.) XP Beads]

    Storage Temperature


    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.


    The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].
    1. What type of sample prep/library construction can I use these Sets for?
    2. How much starting material do I need to use when preparing libraries using the NEBNext® RNA sets?
    3. Do I need any reagents that are not supplied in the kit?
    1. Protocol (E6110) for Use with NEBNext Singleplex (#E7350) or Multiplex (#E7335, #E7500) Oligos for Illumina
    2. Protocol for use with End User Supplied Adaptors & Primers (E6110)
    3. Second Strand cDNA Synthesis
    4. End Repair of cDNA Library