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  • NEBNext® Quick DNA Library Prep Master Mix Set for 454™


    The NEBNext Quick DNA Library Prep Master Mix Set for 454 contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.

    Functional Validation
    Each set of reagents is functionally validated together through construction and sequencing of a DNA library on the 454 platform.

    Lot Control

    The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    NEBNext End Prep Enzyme Mix
    NEBNext End Repair Reaction Buffer10X
    Quick T4 DNA Ligase-20
    NEBNext Sizing Buffer1.2X
    TE Buffer
    Nuclease-free Water-20

    Properties and Usage

    Materials Required but not Supplied

    Agencourt® Ampure® Beads (Beckman Coulter)
    DNA Adaptors or Vectors
    Magnetic Separation Rack (NEB #S1510)

    Storage Temperature



    1. Are NEBNext® products validated in Next Generation Sequencing workflows?


    1. NEBNext End Repair and dA-Tailing (E6090)
    2. Agencourt® Ampure® Beads (Beckman Coulter) Preparation (E6090)
    3. NEBNext Adaptor Ligation (E6090)
    4. NEBNext Small Fragment Removal (E6090)


    The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.