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  • NEBNext® Quick DNA Library Prep Reagent Set for 454™

    E6090 can be substituted for this application

    Discontinued Date

    03/18/2014
    Catalog #SizeConcentrationPriceQtyAdd to Cart
      
    Categories:
    Discontinued Products

    Description

    The NEBNext® Quick DNA Library Prep Reagent Set for 454™ contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.

    Functional Validation
    Each set of reagents is functionally validated together through construction and sequencing of a DNA library on the 454 platform.

    Lot Control

    The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    NEBuffer 2-2010X
    Deoxynucleotide Solution Mix-2010 mM
    T4 DNA Polymerase-203,000 units/ml
    Adenosine-5'-Triphosphate (ATP)10 mM
    T4 Polynucleotide Kinase-2010,000 units/ml
    Taq DNA Polymerase with ThermoPol™ Buffer-205,000 units/ml
    Quick T4 DNA Ligase
    NEBNext Sizing Buffer1.2X
    TE Buffer

    Properties and Usage

    Materials Required but not Supplied

    Agencourt® Ampure® Beads (Beckman Coulter)
    DNA Adaptors or Vectors
    Magnetic Separation Rack (NEB #S1510)

    Storage Temperature

    -20°C

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Manuals

    The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].
    1. NEBNext End Repair and dA-Tailing (E6080)
    2. Agencourt® Ampure® Bead (Beckman Coulter) Preparation (E6080)
    3. NEBNext Adaptor Ligation (E6080)
    4. NEBNext Small Fragment Removal (E6080)