Refer to your specific sample preparation protocol to determine conditions for use.
The NEBNext DNA Library Prep Reagent Set for 454 contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing (1), and for preparation of single stranded DNA for use in high density hybridization arrays (2) or for genomic subtraction hybridization methods (3). Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact OEM@neb.com for further information.
Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a Roche 454 GS FLX Titanium™ System, Roche 454 GS FLX Titanium™ System.
The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page