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  • LongAmp™ Taq PCR Kit

    Description

    The LongAmp Taq PCR Kit features LongAmp Taq DNA Polymerase, which is a unique blend of Taq and Deep VentR ™ DNA Polymerases. The 3´→5´ exonuclease activity of Deep VentR Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). LongAmp Taq DNA Polymerase offers two fold higher fidelity than Taq Polymerase alone. The LongAmp Taq PCR Kit is supplied with LongAmp Taq Reaction Buffer, which is optimized to give highest PCR yields up to 30 kb. The kit can generate a wide range of PCR products, up to 30 kb from plasmid or from human genomic DNA.


    Amplification of specific sequences from human genomic DNA using LongAmp Taq DNA Polymerase. Amplicon sizes are indicated below the gel. Marker M is NEB 1 kb DNA Ladder (NEB #N3232).

    Kit Components

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    Deoxynucleotide (dNTP) Solution Mix-2010 mM
    Nuclease-free Water
    LongAmp® Taq DNA Polymerase-202,500 units/ml
    LongAmp® Taq Reaction Buffer Pack-205X
    Magnesium Sulfate (MgSO4) Solution-20100 mM

    Advantages and Features

    Features

    • Offers two fold higher fidelity than Taq DNA Polymerase alone
    • Allows for robust amplification of PCR products up to 30 kb from low complexity templates and up to 30 kb from difficult templates, like human genomic DNA

    Applications

    • Long Range PCR
    • Colony PCR

    Properties and Usage

    Storage Temperature

    -20°C

    Quality Control

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • PCR Amplification (Master Mix):
      The polymerase is tested in a polymerase chain reaction (PCR) using a control template and specific primers, resulting in the expected product.

    References

    1. Barnes, W.M. (1994). Proc. Natl. Acad. Sci. 91, 2216-2220.
    2. Saiki R.K. et al. (1985). Science. 230, 1350-1354.
    3. Powell, L.M. et al. (1987). Cell. 50, 831-840.
    4. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
    5. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res. 18, 7465.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. What is the recommended enzyme amount when using LongAmp™ Taq DNA Polymerase?
    2. What is the fidelity of the LongAmp™ Taq DNA Polymerase compared to Taq DNA Polymerase?
    3. Can the extension step be carried out at 72°C when using LongAmp™ Taq DNA Polymerase?
    4. What is the extension rate when using LongAmp™ Taq DNA Polymerase?
    5. Why is the product a smear when visualized on an agarose gel?
    6. Can the LongAmp™ kit be used to amplify GC-rich amplicons?
    7. How is OneTaq DNA Polymerase different from LongAmp™ Taq DNA Polymerase?
    8. What type of DNA ends result from a primer extension reaction or a PCR using LongAmp™ Taq DNA Polymerase?
    1. Protocol for LongAmp™ Taq PCR Kit

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