Featured Video
-
Product Information
Chemically competent E. coli cells suitable for high efficiency transformation and protein expression. Highlights
- Enhanced BL21 derivative
- T7 RNA Polymerase in the lac operon - no λ prophage
- Deficient in proteases Lon and OmpT
- Resistant to phage T1 (fhuA2)
- Does not restrict methylated DNA (McrA-, McrBC-, EcoBr-m-, Mrr-)
- B Strain
- Free of animal products
Transformation Efficiency
0.6-1 x 109 cfu/μg pUC19Genotype
fhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11 R(mcr-73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS) endA1 Δ(mcrC-mrr)114::IS10- This product is related to the following categories:
- T7 Expression,
- E. coli Protein Expression Strains Products,
- Protein Expression Products, E. coli Expression Strains Products
- This product can be used in the following applications:
- T7 Expression,
- Peptide Ligation,
- Protein Expression in E. Coli, Protein Expression
-
Protocols, Manuals & Usage
-
Tools & Resources
-
FAQs & Troubleshooting
-
Citations & Technical Literature
-
Product Citation Tool
Additional Citations
- Anton, B.P., Fomenkov, A., Raleigh, E.A. and Berkmen, M. (2016) Complete Genome Sequence of the Engineered Escherichia coli SHuffle Strains and Their Wild-Type Parents Genome Announc; Mar 31;4(2), PubMedID: 27034504, DOI: 10.1128/genomeA.00230-16.
Featured Videos
Other Products You May Be Interested In
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at freezers@neb.com or 1-800-632-5227 x 8.
Powered by Bioz
Choose your country
North America
Europe
Asia-Pacific
Find a Golden Butterfly for a Chance to Win!
Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.