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  • K. lactis GG799 Competent Cells

    Description

    Chemically competent Kluyveromyces lactis cells suitable for transformation with a linearized pKLAC-series expression vector.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    NEB Yeast Transformation Reagent (5 ml)1X

    Properties and Usage

    Storage Temperature

    -80°C

    Shipping Notes

    • Ships on dry ice

    Quality Control

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Sterility (K. lactis) :
      The product is tested for bacterial and/or fungal contamination by a plating assay.
    • Transformation Efficiency:
      The competent cells are tested for transformation efficiency and pass minimum release criteria. Transformation efficiency is defined as the number of colony forming units (cfu) which would be produced by transforming 1 μg of plasmid into a given volume of competent cells.

    Notes

    1. Store Competent Cells at -80°C. Once Thawed, Do Not Re-freeze.
    2. Due to the high transformation efficiency of K. lactis GG799 Competent Cells, plating multiple dilutions of the cell mixture is necessary to ensure formation of plates with distinct single colonies. Growth time should not exceed 5 days as small colonies that lack an integrated expression fragment may form.
    3. Plates containing colonies can be stored at 4°C for up to two weeks.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. Transformation Protocol for K. lactis GG799 Competent Cells (C1001)

    Application Notes

    Always store immediately at -80 C.  Yeast competent cells will quickly lose competency if handled improperly.