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  • Yeast Medium Pack

    Description

    This medium pack includes 12 grams of Yeast Carbon Base and 10 ml of Acetamide.

    The Yeast Carbon Base (YCB) Medium Pack contains sufficient reagents needed to make 1 liter of YCB Agar Medium containing 5 mM acetamide.

    YCB Agar Medium is used for acetamide selection of K. lactis cells that have been transformed with a pKLAC1-based expression vector (NEB #N3740). The medium contains glucose and all nutrients needed to sustain growth of K. lactis GG799 cells except a simple nitrogen source. Only transformed cells can utilize acetamide provided in the medium as a source of nitrogen after it is broken down to ammonia by acetamidase (the product of the amdS gene present in pKLAC1).

    Kit Components

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    Yeast Carbon Base Medium (12g)
    Acetamide solution (sterile) (10 ml)100X

    Properties and Usage

    Storage Temperature

    4°C

    Supporting Documents

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. Protocol I: Yeast Agar Medium with 5 mM acetamide solution (500 ml)
    2. Protocol II: 1 M Tris-HCl Buffer Stock Solution (1 liter)
    Make sure agar is cooled to ~50 C before adding acetamide