• My NEB
  • Print
  • PDF
  • ThermoPol® II (Mg-free) Reaction Buffer Pack


    10X ThermoPol II (Mg-free) Reaction Buffer and supplemental MgSO4 are optimized for use with VentR® and Deep VentR™ DNA Polymerases. This buffer and supplement also provide superior reaction conditions for other thermophilic DNA polymerases, including Taq DNA Polymerase, as well as various other DNA and RNA modifying enzymes. The Mg-free reaction buffer and supplemental MgSO4 are provided for users that require complete control over Mg2+ concentration to optimize their specific application. See individual product descriptions for the recommended buffer for each enzyme, or visit the FAQ page for this product on the NEB web site for a complete list of enzymes for which this buffer is either recommended or compatible.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    Magnesium Sulfate (MgSO4) Solution-20100 mM
    ThermoPol® II (Mg-Free) Reaction Buffer-2010X

    Properties and Usage

    Storage Temperature


    1X Buffer Components

    20mM Tris-HCl
    10mM (NH4)2SO4
    10mM KCl
    0.1% Triton® X-100
    pH 8.8@25°C

    Quality Control

    Quality Assurance Statement

    • This reaction buffer and supplement are free of detectable nucleases.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.


    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. What is supplied with the ThermoPol® II Reaction Buffer pack?
    2. What is the composition of the ThermoPol® II (Mg-free) Reaction Buffer?
    3. Which Polymerases use ThermoPol® II (Mg-free) Reaction Buffer?
    4. How should the NEBuffer be used?
    5. The buffer arrived thawed.  Are the buffer and enzyme still active?