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  • S-adenosylmethionine (SAM)

    Description

    S-adenosyl-methionine (SAM) is included with all of the DNA methylases available from New England Biolabs, Inc. As well as certain restriction enzymes for which it is required. The SAM is prepared in a solution of 0.005 M H2SO4 and 10% ETOH and filtered.

    Properties and Usage

    Storage Temperature

    -20°C

    Quality Control

    Quality Assurance Statement

    • Each lot of SAM is tested in an SssI (CpG) Methylase (NEB #M0226) reaction with λ DNA. The DNA is then challenged with BstUI to confirm methylation.

    Notes

    1. For use of methylation reaction the SAM should be diluted 1:400 to a final concentration of 80μM.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. What is the source of your S-adenosyl-methionine (SAM)?
    2. What is the buffer composition of your S-adenosyl-methionine (SAM)?
    3. Will the buffer from the SAM inhibit my reaction?
    4. What quality controls are performed on your SAM?
    5. Is SAM supplied with enzymes that require it in the reaction buffer?
    6. How stable is SAM in a reaction?
    7. Should I replenish the SAM in my reaction?
    8. How long can I store SAM?