New to Cloning?
There are several methods and techniques available for cloning. Traditionally, cloning has utilized restriction enzymes to excise the DNA of interest, and to linearize a plasmid vector while creating compatible ends. After purification of the insert and vector, both are joined with the activity of a DNA ligase, and the newly-created recombinant vector is used to transform an E. coli host for propagation. PCR has also been used to generate both the vector and insert, which can be joined using a variety of techniques, ranging from standard DNA ligation or enzymatic joining using a recombinase or topoisomerase, to homologous recombination. More recently, DNA assembly techniques have been developed that utilize a mixture of enzymes to assemble DNA fragments in a single tube.
Learn more about the cloning workflow
Whether you are new to cloning, or having difficulties with an existing experiment, NEB offers a wide selection products, tools and resources that can help you be more efficient and successful with your experiments. To get started, choose the step in the cloning workflow below that you are interested in to find recommended products, videos, technical tips and more.
Nucleic acid purification
- Troubleshooting Guide for DNA Purification
- Do’s and Don'ts of Plasmid Minipreps
- Usage Guidelines for the Monarch Plasmid Miniprep Kit when Working with Low Copy Plasmids
- Six Tips for Perfect Gel Extraction
Restriction enzyme digestion
One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at firstname.lastname@example.org. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.
Feature Cloning Resources
|Getting Started with Molecular Cloning
Explore simple tips to improve efficiency in your cloning experiment.
|Troubleshooting Guide for Cloning
Find common problems and solutions associated with cloning experiments.
|Traditional Cloning Quick Guide
Find quick tips for optimization of each step in the cloning workflow.
Find products, protocols and tips for each step of your traditional cloning experiment.
For help with scientific calculations and conversions
BROCHURES & TECHNICAL GUIDES
|Molecular Cloning Technical Guide
Get help with product selection, protocols, tips for optimization and trouble-shooting.
|Reagents & Tools for Molecular
Learn about NEB's recommended products for cloning.
Traditional Cloning refers to the generation of DNA fragments using restriction enzymes, and their subsequent assembly and transformation. The name is derived from the method’s history as the first widely-accepted cloning method. Learn more about the benefits and disadvantages of Traditional Cloning.