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NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 5)

For optimal workflow flexibility, NEBNext Multiplex Oligos are available separately from library preparation kits, providing adaptors and primers for high-yield multiplex Illumina library production. The NEBNext Adaptor has a unique hairpin loop structure that minimizes adaptor-dimer formation, while NEBNext index PCR primers enable index (barcode) incorporation during library amplification.  This fifth set of unique-dual indices (UDIs) includes 96 pre-mixed unique pairs of index primers (i5 and i7), packaged in a single-use 96-well plate with a pierceable foil seal.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

  • Enables complete filtering of index-hopped reads
  • Highly efficient adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing
  • Combine with sets 1, 2, 3 and 4 for 480 barcodes
 
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Catalog # Concentration Size
E6448S Not Applicable 96 reactions
E6448L Not Applicable 384 reactions
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