NEBNext Ultra II RNA Kits offer exceptional yields of high-quality libraries
Smarter, more streamlined RNA library prep with NEBNext®
• Our NEBNext UltraExpress™ RNA Library Prep Kit yields high-quality RNA libraries in 3 hours, following mRNA enrichment or RNA depletion (optional)
• Generate strand-specific or non-stranded RNA libraries from as little as 5 ng input with our NEBNext Ultra™ II RNA Library Prep Kits
• NEBNext Multiplex Oligos enable simple yet powerful NGS sample multiplexing
• Optimized workflows for small RNAs, and single cell and ultra-low input RNA
Streamlined
for Speed
NEBNext® Ultra™ II Reagents & Kits BrochureView
In this brochure, learn more about the benefits of the Ultra II workflow, and NEBNext Ultra II sample preparation products available for sequencing on the Illumina® platform.
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NEBNext For RNA Sample Prep Brochure
The NEBNext for RNA Sample Prep Brochure provides an overview of the NEBNext sample preparation products for sequencing on the Illumina platform, as well as tech tips and workflow overviews.
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Obtain superior NGS library performance with lower input amounts using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina
This technical note offers advances in non-strand-specific RNA library construction in a poly(A) mRNA enrichment workflow.
Read Tech NoteFeatured NEBNext Ultra II RNA products
High-quality, high-yield RNA libraries are critical for RNA sequencing. They enable the analysis of gene expression and regulation, discovery of novel transcripts, study of RNA modifications, and investigation of isoform diversity.
Our extensive range of NEBNext library prep options encompass kits and workflows tailored for rapid processing of samples across a broad input range, including small RNAs, optimizing your workflow efficiency.
NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®
NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®
Includes optimized mixes for directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps). Available with (NEB #E7765) or without (NEB #E7760) sample purification beads.
NEBNext UltraExpress™ RNA Library Prep Kit
NEBNext UltraExpress™ RNA Library Prep Kit
With a 3-hour library prep protocol, the kit enables creation of high-quality RNA libraries in a single day, in conjunction with mRNA enrichment or rRNA depletion kits.
NEBNext® Ultra™ II RNA Library Prep Kit for Illumina®
NEBNext® Ultra™ II RNA Library Prep Kit for Illumina®
This kit generates high yields of high-quality libraries from lower input amounts and fewer PCR cycles. Available with (NEB #E7775) or without (NEB #E7770) sample purification beads.
NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs)
NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs)
The NEBNext hairpin loop structure of the adaptor minimizes dimer formation; Set 1 of 5 UDI primer pairs for i5 and i7 indexes are pre-mixed in a convenient 96-well plate.
This content is covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.
