Nucleic acid purification is an essential step in many molecular biology workflows, including enzyme digests, transformation, electrophoresis, RT-PCR, RT-qPCR and library preparation. Monarch kits enable quick and easy purification of high-quality DNA and RNA, suitable for direct use in a variety of downstream applications. Recover pure, intact DNA and RNA in minutes with fast, user-friendly protocols and optimized buffer systems, and focus your time on the experiments that will drive your research forward.

Monarch kits are all designed with sustainability in mind; whenever possible, kits and components are made with significantly less plastic and are packaged with responsibly-sourced, recyclable packaging.

MonarchKits_DNA_RNA The Monarch Total RNA Miniprep Kit is optimized for use with a wide variety of sample types, so that one convenient kit can serve your RNA purification needs.

Monarch DNA purification kits are available for plasmid miniprep, reaction cleanup, and gel extraction. Monarch DNA columns are designed without a retaining ring, eliminating the potential for buffer carryover and enabling low-volume elution.

4 Reasons to Choose Monarch


A simple choice for your RNA purification – one kit for highly-pure RNA

  • Use with multiple sample types, including blood, cells, tissues and tough-to-lyse samples

  • Effectively purify RNA of all sizes, including small RNA (<200 nt)

  • Efficiently remove genomic DNA with included gDNA Removal Columns and DNase I

Highly pure DNA ready for use in multiple downstream applications

  • Easily purify high-quality DNA with excellent yields from bacterial cultures, agarose gels and enzymatic reactions

  • Elute in low volumes for highly-concentrated DNA

  • Eliminate buffer retention and carryover contamination with our columns that do not contain retaining rings

  • Save time with fast, user-friendly protocols and optimized buffer systems

  • Take advantage of columns designed for easy labeling and handling

A more sustainable purification experience

  • Kit configurations are optimized for minimal material consumption

  • Decrease your plastic usage with custom, thin-walled columns and collection tubes

  • Kits are packaged with responsibly-sourced and recyclable packaging and printed with water and soy-based ink

  • Reuse your Monarch box and share your upcycle with #NEBMonarchUpcycle

Pure Value


Available kits include:

Monarch Total RNA Miniprep Kit 
Extract and purify up to 100 µg of high-quality RNA from a variety of sample types with a single, convenient kit. DNase I, gDNA removal columns, Proteinase K, and a protection reagent are all included. Monarch-purified RNA is ready to use in RT-PCR, RT-qPCR, RNA-seq and other applications.

Monarch Plasmid Miniprep Kit
This kit employs familiar cell resuspension, alkaline lysis and neutralization steps, with the additional benefit of color indicators to monitor completion. Elute in lower volumes for more concentrated, highly pure DNA samples.


Monarch DNA Gel Extraction Kit
Rapidly purify up to 5 μg of concentrated, high-quality DNA from your agarose gels, with no need to adjust pH. Elute in as little as 6 μl for a more concentrated sample. Enjoy high yields and minimal hands on time.


Monarch PCR & DNA Cleanup Kit (5 μg)
Purify DNA from a variety of enzymatic reactions, including PCR, restriction digestion, ligation and reverse transcription. Also isolate small DNA including oligos with a slight protocol modification.

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