Cell cultures are often analyzed for gene expression or treatment responses as a proxy for a living organism. Traditionally, RNA is extracted and purified from treated cells via column-based or chemical methods. The Luna Cell Ready Lysis Module is a fast, convenient, and sensitive alternative for evaluating RNA expression levels without performing traditional RNA extraction steps. The module coordinates cell lysis, RNA release, and genomic DNA removal simultaneously, resulting in cell lysate ready for direct RT-qPCR analysis with Luna Universal One-Step RT-qPCR kits. Similar to other Luna products, the lysis buffer includes an inert blue tracking dye for visual assistance throughout the workflow.
This table lists cell lines that are compatible with the Luna Cell Ready Lysis Module. It includes results seen using a 5-log dilution series (100,000 - 10 cells per 50 µl lysis) and subsequent detection of 1 µl of lysate in a 20 µl one-step RT-qPCR reaction. For each cell line, linear results are indicated in the final column. In addition, some insect cell lines have been tested and are compatible.
Table 1: Validated Cell Lines
|Cell line||Property||Species||Cell number per 50 μl lysate|
|A549||Adherent||H. sapiens, Lung, carcinoma||10 - 100,000|
|HEK293||Adherent||H. sapiens, Kidney||10 - 100,000|
|HeLa||Adherent||H. sapiens, Cervix, adenocarcinoma||10 - 100,000|
|HepG2||Adherent||H. sapiens, Liver, carcinoma||10 - 1,000|
|NCI-H460||Adherent||H. sapiens, Lung, carcinoma||10 - 100,000|
|SK-N-SH||Adherent||H. sapiens, Brain, Neuroblastoma||10 - 10,000|
|U2Os||Adherent||H. sapiens, Bone, osteosarcoma||10 - 100,000|
|Jurkat||Suspension||H. sapiens, T lymphocyte, leukemia||10 - 100,000|
|K-562||Suspension||H. sapiens, Lymphoblast, leukemia||10 - 100,000|
The following reagents are supplied with this product:
|NEB #||Component Name||Component #||Stored at (°C)||Amount||Concentration|
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