The Luna RT-qPCR kits contain a novel, in silico-designed reverse transcriptase (RT) engineered for improved performance. Both the Luna WarmStart Reverse Transcriptase and Hot Start Taq DNA Polymerase, included in these kits, utilize a temperature-sensitive, reversible aptamer, which inhibits activity below 45°C. This enables room temperature reaction setup and prevents undesired non-specific activity. Furthermore, the WarmStart RT has increased thermostability, improving performance at higher reaction temperatures.
Improve performance with our novel, thermostable reverse transcriptase
Luna WarmStart Reverse Transcriptase exhibits exceptional sensitivity, reproducibility and RT-qPCR performance
RT-qPCR targeting human GAPDH was performed using the Luna Universal One-Step RT-qPCR Kit, featuring Luna WarmStart Reverse Transcriptase, over an 8-log range of input template concentrations (0.1 pg – 1 μg Jurkat total RNA) with 8 replicates at each concentration. NTC = non-template control.
Achieve better control and better results: WarmStart Luna RT paired with Hot Start Taq
WarmStart Luna RT prevents spurious amplification resulting from room-temperature pre-incubation
RT-qPCR targeting human ribosomal protein L32 was performed before and after a 24-hour incubation at room temperature, with triplicate reactions for a 5-log range of input human (Jurkat) total RNA and a non-template control. The Luna Universal One-Step RT-qPCR Kit featuring Luna WarmStart Reverse Transcriptase exhibited robust performance and no detectible non-template amplification, either with or without a 24 hour 25°C pre-incubation, while the ABI 1-Step Kit, featuring a non-WarmStart reverse transcriptase, exhibited significant non-template amplification.
Greater thermostability means greater versatility
The increased thermostability of WarmStart Luna Reverse Transcriptase improves performance at increased reaction temperatures
RT-qPCR experiments targeting human ribosomal protein L32 RNA were performed in triplicate over a 5-log range of input human (Jurkat) total RNA (5 pg – 50 ng) using an initial 10 min RT step performed at 50°C – 60°C, as indicated.
A. Luna WarmStart Reverse Transcriptase (recommended incubation temperature: 55°C) exhibited rapid Cq values (bar graph) and robust RT-qPCR performance (amplification plots) at each temperature, indicating that efficient reverse transcription was not perturbed by reaction temperature alterations.
B. In contrast, a commercially available MMLV (recommended incubation temperature: 50°C) exhibited delayed (increased) Cq values, poorer performance, and loss of low-input detection at elevated temperatures, consistent with loss of RT activity.
Available products include:
|Luna Universal qPCR Master Mix
Rapid, sensitive, and precise dye-based qPCR detection and quantitation of target DNA and cDNA sequences
|Luna Universal Probe qPCR Master Mix
Rapid, sensitive, and precise probe-based qPCR detection and quantitation of target DNA and cDNA sequences
|Luna Universal One-Step RT-qPCR Kit
Includes everything you need for rapid, sensitive, and precise dye-based qPCR detection and quantitation of RNA targets
|Luna Universal Probe One-Step RT-qPCR Kit
Includes everything you need for rapid, sensitive, and precise probe-based qPCR detection and quantitation of RNA targets
One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at firstname.lastname@example.org. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.
SYBR® is a registered trademark of Molecular Probes, Inc.
ARRAYSCRIPT™ is a trademark of Thermo Fisher Scientific,Inc.