University of Wisconsin-Madison NEBnow®

Available in this freezer

NEB # Buffers Size Stockroom #
B9000S BSA, Molecular Biology Grade 12 mg B9000S
B0202S T4 DNA Ligase Reaction Buffer 6 ml B0202S
NEB # DNA Modifying Enzymes and Cloning Technologies Size Stockroom #
E2611S Gibson Assembly® Master Mix 10 reactions E2611S
M0212S Klenow Fragment (3'→5' exo-) 200 units M0212S
E2621S NEBuilder® HiFi DNA Assembly Master Mix 10 reactions E2621S
M2200S Quick Ligation™ Kit 30 reactions M2200S
M0202L T4 DNA Ligase 100,000 units M0202L
M0202S T4 DNA Ligase 20,000 units M0202S
NEB # Epigenetics Size Stockroom #
R0176S DpnI 1,000 units R0176S
NEB # Markers & Ladders Size Stockroom #
N3232S 1 kb DNA Ladder 0.2 ml N3232S
N3232L 1 kb DNA Ladder 1 ml N3232L
N3231L 100 bp DNA Ladder 0.5 ml N3231L
N3231S 100 bp DNA Ladder 0.1 ml N3231S
N0552L Quick-Load® Purple 1 kb DNA Ladder 3.75 ml N0552L
N0552S Quick-Load® Purple 1 kb DNA Ladder 1.25 ml N0552S
N0551L Quick-Load® Purple 100 bp DNA Ladder 3.75 ml N0551L
N0551S Quick-Load® Purple 100 bp DNA Ladder 1.25 ml N0551S
NEB # Nucleic Acid Purification Size Stockroom #
T1020L Monarch® DNA Gel Extraction Kit 250 preps T1020L
T1020S Monarch® DNA Gel Extraction Kit 50 preps T1020S
T3010S Monarch® Genomic DNA Purification Kit 50 preps T3010S
T3010L Monarch® Genomic DNA Purification Kit 150 preps T3010L
T1030S Monarch® PCR & DNA Cleanup Kit (5 μg) 50 preps T1030S
T1030L Monarch® PCR & DNA Cleanup Kit (5 μg) 250 preps T1030L
T1010L Monarch® Plasmid Miniprep Kit  250 preps T1010L
T1010S Monarch® Plasmid Miniprep Kit  50 preps T1010S
NEB # PCR, Polymerases & Amplification Technologies Size Stockroom #
M0210S DNA Polymerase I, Large (Klenow) Fragment 200 units M0210S
E3005S Luna® Universal One-Step RT-qPCR Kit 200 reactions E3005S
M3003S Luna® Universal qPCR Master Mix 200 rxn (2 x 1 ml) M3003S
E3010S LunaScript RT SuperMix Kit 25 reactions E3010S
M0480L OneTaq® DNA Polymerase 1,000 units M0480L
M0480S OneTaq® DNA Polymerase 200 units M0480S
M0530L Phusion® High-Fidelity DNA Polymerase 500 units M0530L
M0530S Phusion® High-Fidelity DNA Polymerase 100 units M0530S
M0531S Phusion® High-Fidelity PCR Master Mix with HF Buffer 100 reactions (50 µl vol) M0531S
M0491L Q5® High-Fidelity DNA Polymerase 500 units M0491L
M0491S Q5® High-Fidelity DNA Polymerase 100 units M0491S
M0494S Q5® Hot Start High-Fidelity 2X Master Mix 100 rxn (2 x 1.25 ml) M0494S
M0203S T4 DNA Polymerase 150 units M0203S
M0273L Taq DNA Polymerase with Standard Taq Buffer 2,000 units M0273L
M0273S Taq DNA Polymerase with Standard Taq Buffer 400 units M0273S
M0267S Taq DNA Polymerase with ThermoPol® Buffer 400 units M0267S
M0267L Taq DNA Polymerase with ThermoPol® Buffer 2,000 units M0267L
NEB # Protein Tools Size Stockroom #
P8107S Proteinase K, Molecular Biology Grade 2 x 1 ml vials P8107S
NEB # Restriction Endonucleases Size Stockroom #
R3552S AgeI-HF® 300 units R3552S
R0558S AscI 500 units R0558S
R3136S BamHI-HF® 10,000 units R3136S
R0144S BglII 2,000 units R0144S
R3101S EcoRI-HF® 10,000 units R3101S
R3104S HindIII-HF® 10,000 units R3104S
R3142S KpnI-HF® 4,000 units R3142S
R3131S NheI-HF® 1,000 units R3131S
R3189S NotI-HF® 500 units R3189S
R3189L NotI-HF® 2,500 units R3189L
R3122S ScaI-HF® 1,000 units R3122S
R3133S SpeI-HF® 500 units R3133S
R0145S XbaI 3,000 units R0145S
R0146S XhoI 5,000 units R0146S
NEB # RNA Reagents Size Stockroom #
T2010S Monarch® Total RNA Miniprep Kit 50 preps T2010S
M0289S Antarctic Phosphatase 1,000 units M0289S
N0446S Deoxynucleotide (dNTP) Solution Set 25 µmol of each N0446S
T2050L Monarch® RNA Cleanup Kit (500 μg) 100 preps T2050L
T2050S Monarch® RNA Cleanup Kit (500 μg) 10 preps T2050S
M0525S Quick CIP 1,000 units M0525S
M0371S Shrimp Alkaline Phosphatase (rSAP) 500 units M0371S
M0201S T4 Polynucleotide Kinase 500 units M0201S