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University of Wisconsin-Madison NEBnow®

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NEB # Buffers Size Stockroom #
B9000S BSA, Molecular Biology Grade 12 mg B9000S
B7204S CutSmart® Buffer 5 ml B7204S
B7024S Gel Loading Dye, Purple (6X) 4 ml B7024S
NEB # DNA Modifying Enzymes Size Stockroom #
E2611S Gibson Assembly® Master Mix 10 reactions E2611S
M0212S Klenow Fragment (3'→5' exo-) 200 units M0212S
E2621S NEBuilder® HiFi DNA Assembly Master Mix 10 reactions E2621S
E2621L NEBuilder® HiFi DNA Assembly Master Mix 50 reactions E2621L
M2200S Quick Ligation™ Kit 30 reactions M2200S
M0202S T4 DNA Ligase 20,000 units M0202S
M0202L T4 DNA Ligase 100,000 units M0202L
M0208S Taq DNA Ligase 2,000 units M0208S
M0208L Taq DNA Ligase 10,000 units M0208L
NEB # Epigenetics Size Stockroom #
R0176S DpnI 1,000 units R0176S
R0176L DpnI 5,000 units R0176L
NEB # Markers & Ladders Size Stockroom #
N3232S 1 kb DNA Ladder 0.2 ml N3232S
N3232L 1 kb DNA Ladder 1 ml N3232L
N3200S 1 kb Plus DNA Ladder 0.1 ml N3200S
N3200L 1 kb Plus DNA Ladder 0.5 ml N3200L
N3231L 100 bp DNA Ladder 0.5 ml N3231L
N3231S 100 bp DNA Ladder 0.1 ml N3231S
N0552S Quick-Load® Purple 1 kb DNA Ladder 1.25 ml N0552S
N0550S Quick-Load® Purple 1 kb Plus DNA Ladder 1.25 ml N0550S
N0551S Quick-Load® Purple 100 bp DNA Ladder 1.25 ml N0551S
NEB # Nucleic Acid Purification Size Stockroom #
T1020S Monarch® DNA Gel Extraction Kit 50 preps T1020S
T1030S Monarch® PCR & DNA Cleanup Kit (5 μg) 50 preps T1030S
T1010S Monarch® Plasmid Miniprep Kit  50 preps T1010S
NEB # PCR, Polymerases & Amplification Technologies Size Stockroom #
M0210S DNA Polymerase I, Large (Klenow) Fragment 200 units M0210S
E3005S Luna® Universal One-Step RT-qPCR Kit 200 reactions E3005S
M3003S Luna® Universal qPCR Master Mix 200 rxn (2 x 1 ml) M3003S
M0480S OneTaq® DNA Polymerase 200 units M0480S
M0480L OneTaq® DNA Polymerase 1,000 units M0480L
M0530S Phusion® High-Fidelity DNA Polymerase 100 units M0530S
M0530L Phusion® High-Fidelity DNA Polymerase 500 units M0530L
M0491L Q5® High-Fidelity DNA Polymerase 500 units M0491L
M0491S Q5® High-Fidelity DNA Polymerase 100 units M0491S
M0203S T4 DNA Polymerase 150 units M0203S
M0273S Taq DNA Polymerase with Standard Taq Buffer 400 units M0273S
M0273L Taq DNA Polymerase with Standard Taq Buffer 2,000 units M0273L
M0267L Taq DNA Polymerase with ThermoPol® Buffer 2,000 units M0267L
M0267S Taq DNA Polymerase with ThermoPol® Buffer 400 units M0267S
NEB # Restriction Endonucleases Size Stockroom #
R3136S BamHI-HF® 10,000 units R3136S
R3539S BbsI-HF® 300 units R3539S
R0144S BglII 2,000 units R0144S
R0580L BsmBI 1,000 units R0580L
R3101S EcoRI-HF® 10,000 units R3101S
R3142S KpnI-HF® 4,000 units R3142S
R3589S MfeI-HF® 500 units R3589S
R0111S NdeI 4,000 units R0111S
R3131S NheI-HF® 1,000 units R3131S
R3189L NotI-HF® 2,500 units R3189L
R3189S NotI-HF® 500 units R3189S
R3156S SacI-HF® 2,000 units R3156S
R3133S SpeI-HF® 500 units R3133S
R3182S SphI-HF® 500 units R3182S
R0145S XbaI 3,000 units R0145S
R0146S XhoI 5,000 units R0146S
R0180S XmaI 500 units R0180S
NEB # RNA Reagents Size Stockroom #
T2010S Monarch® Total RNA Miniprep Kit 50 preps T2010S
M0289S Antarctic Phosphatase 1,000 units M0289S
N0447S Deoxynucleotide (dNTP) Solution Mix 8 µmol of each N0447S
N0447L Deoxynucleotide (dNTP) Solution Mix 40 µmol of each N0447L
N0446S Deoxynucleotide (dNTP) Solution Set 25 µmol of each N0446S
M0303S DNase I (RNase-free) 1,000 units M0303S
T2030S Monarch® RNA Cleanup Kit (10 μg) 10 preps T2030S
T2040S Monarch® RNA Cleanup Kit (50 μg) 10 preps T2040S
T2050S Monarch® RNA Cleanup Kit (500 μg) 10 preps T2050S
M0314S RNase Inhibitor, Murine 3,000 units M0314S
M0314L RNase Inhibitor, Murine 15,000 units M0314L
M0371S Shrimp Alkaline Phosphatase (rSAP) 500 units M0371S
M0201S T4 Polynucleotide Kinase 500 units M0201S
M0242L T4 RNA Ligase 2, truncated 10,000 units M0242L

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