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University of Wisconsin-Madison NEBnow®

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Inventory as of 7/3/2020 2:42 AM

NEB # Buffers Size Quantity Available
B9000S BSA, Molecular Biology Grade 12 mg 4
B7204S CutSmart® Buffer 5 ml 2
B7024S Gel Loading Dye, Purple (6X) 4 ml 2
NEB # DNA Modifying Enzymes and Cloning Technologies Size Quantity Available
E2611S Gibson Assembly® Master Mix 10 reactions 2
M0212S Klenow Fragment (3'→5' exo-) 200 units 2
E2621L NEBuilder® HiFi DNA Assembly Master Mix 50 reactions 2
E2621S NEBuilder® HiFi DNA Assembly Master Mix 10 reactions 2
M2200S Quick Ligation™ Kit 30 reactions 2
M0202S T4 DNA Ligase 20,000 units 4
M0202L T4 DNA Ligase 100,000 units 4
M0208S Taq DNA Ligase 2,000 units 2
M0208L Taq DNA Ligase 10,000 units 2
NEB # Epigenetics Size Quantity Available
R0176S DpnI 1,000 units 3
R0176L DpnI 5,000 units 3
NEB # Markers & Ladders Size Quantity Available
N3232L 1 kb DNA Ladder 1 ml 2
N3232S 1 kb DNA Ladder 0.2 ml 2
N3200S 1 kb Plus DNA Ladder 0.1 ml 2
N3200L 1 kb Plus DNA Ladder 0.5 ml 2
N3231L 100 bp DNA Ladder 0.5 ml 2
N3231S 100 bp DNA Ladder 0.1 ml 2
N0552S Quick-Load® Purple 1 kb DNA Ladder 1.25 ml 2
N0550S Quick-Load® Purple 1 kb Plus DNA Ladder 1.25 ml 2
N0551S Quick-Load® Purple 100 bp DNA Ladder 1.25 ml 1
NEB # Nucleic Acid Purification Size Quantity Available
T1020S Monarch® DNA Gel Extraction Kit 50 preps 1
T3010S Monarch® Genomic DNA Purification Kit 50 preps 2
T3010L Monarch® Genomic DNA Purification Kit 150 preps 1
T1030S Monarch® PCR & DNA Cleanup Kit (5 μg) 50 preps 1
T1010S Monarch® Plasmid Miniprep Kit  50 preps 1
NEB # PCR, Polymerases & Amplification Technologies Size Quantity Available
M0210S DNA Polymerase I, Large (Klenow) Fragment 200 units 2
E3005S Luna® Universal One-Step RT-qPCR Kit 200 reactions 2
M3003S Luna® Universal qPCR Master Mix 200 rxn (2 x 1 ml) 2
M0480L OneTaq® DNA Polymerase 1,000 units 2
M0480S OneTaq® DNA Polymerase 200 units 2
M0530L Phusion® High-Fidelity DNA Polymerase 500 units 3
M0530S Phusion® High-Fidelity DNA Polymerase 100 units 3
M0491L Q5® High-Fidelity DNA Polymerase 500 units 3
M0491S Q5® High-Fidelity DNA Polymerase 100 units 2
M0203S T4 DNA Polymerase 150 units 2
M0273L Taq DNA Polymerase with Standard Taq Buffer 2,000 units 1
M0273S Taq DNA Polymerase with Standard Taq Buffer 400 units 2
M0267L Taq DNA Polymerase with ThermoPol® Buffer 2,000 units 1
M0267S Taq DNA Polymerase with ThermoPol® Buffer 400 units 3
NEB # Restriction Endonucleases Size Quantity Available
R3136S BamHI-HF® 10,000 units 2
R3539S BbsI-HF® 300 units 2
R0144S BglII 2,000 units 2
R3101S EcoRI-HF® 10,000 units 2
R3142S KpnI-HF® 4,000 units 2
R3589S MfeI-HF® 500 units 2
R0111S NdeI 4,000 units 1
R3131S NheI-HF® 1,000 units 2
R3189S NotI-HF® 500 units 2
R3189L NotI-HF® 2,500 units 2
R3156S SacI-HF® 2,000 units 2
R3133S SpeI-HF® 500 units 2
R3182S SphI-HF® 500 units 2
R0145S XbaI 3,000 units 2
R0146S XhoI 5,000 units 2
R0180S XmaI 500 units 2
NEB # RNA Reagents Size Quantity Available
T2010S Monarch® Total RNA Miniprep Kit 50 preps 1
M0289S Antarctic Phosphatase 1,000 units 2
N0447S Deoxynucleotide (dNTP) Solution Mix 8 µmol of each 2
N0447L Deoxynucleotide (dNTP) Solution Mix 40 µmol of each 2
N0446S Deoxynucleotide (dNTP) Solution Set 25 µmol of each 2
M0303S DNase I (RNase-free) 1,000 units 2
T2030S Monarch® RNA Cleanup Kit (10 μg) 10 preps 1
T2040S Monarch® RNA Cleanup Kit (50 μg) 10 preps 1
T2050S Monarch® RNA Cleanup Kit (500 μg) 10 preps 1
M0525S Quick CIP 1,000 units 1
M0314S RNase Inhibitor, Murine 3,000 units 2
M0314L RNase Inhibitor, Murine 15,000 units 2
M0371S Shrimp Alkaline Phosphatase (rSAP) 500 units 2
M0201S T4 Polynucleotide Kinase 500 units 3
M0242L T4 RNA Ligase 2, truncated 10,000 units 2

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