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University of Wisconsin-Madison NEBnow®

My NEB Freezer Location and Hours

Location:
Department of Biotechnology Room 2221
425 Henry Mall
Madison, WI 53706

Hours:
NEBnow Kiosk, 24 x 7 access

Contact:
Janet Taggart
Phone:
steffen@nutrisci.wisc.edu

NEBNow Purchasing From Freezer
NEBnow® Purchasing from Your NEB Product Freezer

Learn how to purchase products from an NEBnow freezer. It’s as quick and easy as 1, 2, 3…

dames_deboe
My NEB Account Manager

James DeBoe
Phone: (978) 778-6011
jdeboe@neb.com

Since 2017, James has worked with customers in Indiana, Michigan, Ohio and Wisconsin, providing them with the resources and technical support necessary to successfully conduct their research, develop products, and advance science. Outside of his sales role, James enjoys supporting programs that develop strong, resourceful, and confident young men through a variety of coaching activities, including baseball and football.

Learn more about your NEBnow freezer


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Products Available in this Freezer


Inventory is replenished every Wednesday. See below for the regularly stocked product list and their estimated availability as of 12/8/2021 1:44 AM.

Are you interested having in an NEB product added to the regularly stocked inventory? Please tell us which product(s) and we'll contact your NEB Account Manager about your request.

NEB # Buffers Size Quantity Available
B9000S BSA, Molecular Biology Grade 12 mg 4
B7204S CutSmart® Buffer 5 ml 2
B7024S Gel Loading Dye, Purple (6X) 4 ml 2
NEB # DNA Assembly Cloning and Mutagenesis Kits Size Quantity Available
E2611S Gibson Assembly® Master Mix 10 reactions 2
E2621L NEBuilder® HiFi DNA Assembly Master Mix 50 reactions 2
E2621S NEBuilder® HiFi DNA Assembly Master Mix 10 reactions 2
NEB # DNA Modifying Enzymes and Cloning Technologies Size Quantity Available
M0289S Antarctic Phosphatase 1,000 units 2
M0212S Klenow Fragment (3'→5' exo-) 200 units 2
M2200S Quick Ligation™ Kit 30 reactions 2
M0371S Shrimp Alkaline Phosphatase (rSAP) 500 units 2
M0202L T4 DNA Ligase 100,000 units 4
M0202S T4 DNA Ligase 20,000 units 3
M0201S T4 Polynucleotide Kinase 500 units 3
M0208S Taq DNA Ligase 2,000 units 2
M0208L Taq DNA Ligase 10,000 units 2
NEB # Epigenetics Size Quantity Available
R0176L DpnI 5,000 units 3
R0176S DpnI 1,000 units 3
NEB # Markers & Ladders Size Quantity Available
N3232S 1 kb DNA Ladder 200 gel lanes (0.2 ml) 2
N3232L 1 kb DNA Ladder 1,000 gel lanes (1 ml) 2
N3200L 1 kb Plus DNA Ladder 1,000 gel lanes (0.5 ml) 2
N3200S 1 kb Plus DNA Ladder 200 gel lanes (0.1 ml) 2
N3231L 100 bp DNA Ladder 500 gel lanes (0.5 ml) 2
N3231S 100 bp DNA Ladder 100 gel lanes (0.1 ml) 2
N0552S Quick-Load® Purple 1 kb DNA Ladder 125 gel lanes (1.25 ml) 2
N0550S Quick-Load® Purple 1 kb Plus DNA Ladder 250 gel lanes (1.25 ml) 2
N0551S Quick-Load® Purple 100 bp DNA Ladder 125 gel lanes (1.25 ml) 2
NEB # Nucleic Acid Purification Size Quantity Available
T2010S Monarch® Total RNA Miniprep Kit 50 preps 1
T2030S Monarch® RNA Cleanup Kit (10 μg) 10 preps 1
T2040S Monarch® RNA Cleanup Kit (50 μg) 10 preps 2
T1020S Monarch® DNA Gel Extraction Kit 50 preps 1
T3010S Monarch® Genomic DNA Purification Kit 50 preps 2
T3010L Monarch® Genomic DNA Purification Kit 150 preps 1
T1030S Monarch® PCR & DNA Cleanup Kit (5 μg) 50 preps 1
T1010S Monarch® Plasmid Miniprep Kit  50 preps 1
T2050S Monarch® RNA Cleanup Kit (500 μg) 10 preps 1
NEB # PCR, Polymerases & Amplification Technologies Size Quantity Available
N0447S Deoxynucleotide (dNTP) Solution Mix 8 µmol 2
N0447L Deoxynucleotide (dNTP) Solution Mix 40 µmol 2
N0446S Deoxynucleotide (dNTP) Solution Set 25 µmol 2
M0210S DNA Polymerase I, Large (Klenow) Fragment 200 units 2
E3005S Luna® Universal One-Step RT-qPCR Kit 200 reactions 2
M3003S Luna® Universal qPCR Master Mix 200 reactions 2
M0480L OneTaq® DNA Polymerase 1,000 units 2
M0480S OneTaq® DNA Polymerase 200 units 2
M0530S Phusion® High-Fidelity DNA Polymerase 100 units 3
M0530L Phusion® High-Fidelity DNA Polymerase 500 units 3
M0491S Q5® High-Fidelity DNA Polymerase 100 units 2
M0491L Q5® High-Fidelity DNA Polymerase 500 units 3
M0314L RNase Inhibitor, Murine 15,000 units 2
M0314S RNase Inhibitor, Murine 3,000 units 2
M0203S T4 DNA Polymerase 150 units 2
M0273S Taq DNA Polymerase with Standard Taq Buffer 400 units 2
M0273L Taq DNA Polymerase with Standard Taq Buffer 2,000 units 1
M0267S Taq DNA Polymerase with ThermoPol® Buffer 400 units 3
M0267L Taq DNA Polymerase with ThermoPol® Buffer 2,000 units 1
NEB # Restriction Endonucleases Size Quantity Available
R3136S BamHI-HF® 10,000 units 2
R3539S BbsI-HF® 300 units 2
R0144S BglII 2,000 units 2
R3101S EcoRI-HF® 10,000 units 2
R3142S KpnI-HF® 4,000 units 2
R3589S MfeI-HF® 500 units 2
R0111S NdeI 4,000 units 2
R3131S NheI-HF® 1,000 units 2
R3189L NotI-HF® 2,500 units 2
R3189S NotI-HF® 500 units 2
R3156S SacI-HF® 2,000 units 2
R3133S SpeI-HF® 500 units 2
R3182S SphI-HF® 500 units 2
R0145S XbaI 3,000 units 2
R0146S XhoI 5,000 units 2
R0180S XmaI 500 units 2
NEB # RNA Reagents Size Quantity Available
M0303S DNase I (RNase-free) 1,000 units 2
M0525S Quick CIP 1,000 units 2
M0242L T4 RNA Ligase 2, truncated 10,000 units 2

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Special Ordering to this Freezer


Looking for a product not currently stocked?

Place an NEBnow special order for any catalog product and save on freight fees. Special orders are consolidated and delivered to the freezer every Wednesday at no additional charge.*


How to place a special order with NEBnow:

1. Login and tap Place Special Orders.

2. Type NEB product number and then tap product to select it.

3. Tap Add to Order to add to cart.

4. To add another product, tap Clear and repeat steps 2 and 3 above.

5. To checkout and finalize special order, tap Confirm.


How to pick up a special order:

1. Login and tap Special Order Pickup.

2. Remove product from freezer without scanning and tap Exit or New Activity.



* Special orders placed before midnight on Monday will be available for pickup at the freezer on Wednesday


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NEBnow FAQs


Find the answers to some common questions below. Still have a question? Please contact NEB Customer Service by telephone (1-800-632-5227) or email (freezers@neb.com).

Registration

How do I gain access to the NEBnow Freezer?

You can request access by completing an NEBnow registration form. Click Register for Access under My NEB Freezer Location and Hours.

When will I receive access to the NEBnow Freezer?

NEB will receive your request and will verify your information with your Institutional Freezer Coordinator. Once authorized, you will receive an email with your access information. You should receive this email within 2 business days of submitting your request.

How do I change my user registration information?

To change or update your contact information, please contact NEB Customer Service by telephone (1-800-632-5227) or email (freezers@neb.com)


Scanning

What if my product cannot be scanned?

If you have difficulty scanning your product’s barcode, you can select the Manual Entry button, which can be found on any of the product screens. Type in the product code, and the product will be added to your running order.

Do I have to scan each individual product that I remove from the freezer?

Yes, each product removed or returned to the freezer must be scanned. Each product you scan will be added to or removed from your order. You will have the opportunity to review and confirm your order prior to checkout.

What is a special order?

Participating freezers allow for the purchase of NEB catalog products not stocked in the freezer. Special orders are consolidated delivered to the freezer on the designated restocking day. See Learn more about your NEBnow freezer to confirm your freezer's participation.


Freezer restocking

How often are the NEBnow freezer products replenished?

NEBnow freezer inventory is replenished on a regular basis. Find your freezer's restocking day by viewing Products Available in this Freezer.

When restocking the freezer, what if the shipping/picking list barcode does not scan?

If the picking list barcode does not scan, please contact NEBnow Customer Service by telephone (1-800-632-5227) or email (freezers@neb.com).


Product

How do I locate the product I want in the freezer?

Refer to the map located on the freezer door to locate the tray in which your product is located.

What is a “Special Order"?

Participating freezers allow for the purchase of NEB catalog products not stocked in the freezer. Special orders are consolidated and delivered to the freezer on the designated restocking day. See Learn more about your NEBnow freezer to confirm your freezer's participation.

How do I know what products are available fore special order?

All NEB catalog products are available for Special Order. Select the Special Order button on the home screen to browse NEB products.

How do I place a special order from the website?

At this time, all special orders need to be initiated at participating freezers. See Learn more about your NEBnow freezer for instruction on how to place a special order.


Password

What if I forget my NEBnow PIN?

After three failed attempts of logging into the NEBnow Freezer, you will receive an email providing you with a new PIN. Alternatively, you may contact NEB Customer Service by telephone (1-800-632-5227) or email (freezers@neb.com) for support.


Other

What should I do if the freezer appears not to be cold enough or not holding its temperature?

Please contact your local Institutional Freezer Coordinator if you notice problems with the freezer.