University of Nebraska Medical Center

Available in this freezer

NEB # Buffers Size Stockroom #
B9000S BSA, Molecular Biology Grade 12 mg B9000S
NEB # DNA Modifying Enzymes & Cloning Technologies Size Stockroom #
M0367S Blunt/TA Ligase Master Mix 50 reactions M0367S
M0293S Exonuclease I (E. coli) 3,000 units M0293S
M0370S Instant Sticky-end Ligase Master Mix 50 reactions M0370S
E2621S NEBuilder® HiFi DNA Assembly Master Mix 10 reactions E2621S
M0530L Phusion® High-Fidelity DNA Polymerase 500 units M0530L
M0530S Phusion® High-Fidelity DNA Polymerase 100 units M0530S
E0552S Q5® Site-Directed Mutagenesis Kit (Without Competent Cells) 10 reactions E0552S
M2200S Quick Ligation™ Kit 30 reactions M2200S
M0202S T4 DNA Ligase 20,000 units M0202S
M0202M T4 DNA Ligase 100,000 units M0202M
NEB # Epigenetics Size Stockroom #
R0176S DpnI 1,000 units R0176S
R3138S SalI-HF® 2,000 units R3138S
NEB # Markers & Ladders Size Stockroom #
N3232S 1 kb DNA Ladder 0.2 ml N3232S
N3231S 100 bp DNA Ladder 0.1 ml N3231S
N0552S Quick-Load® Purple 1 kb DNA Ladder 1.25 ml N0552S
N0550S Quick-Load® Purple 1 kb Plus DNA Ladder 1.25 ml N0550S
N0551S Quick-Load® Purple 100 bp DNA Ladder 1.25 ml N0551S
P7704S Unstained Protein Standard, Broad Range (10-200 kDa) 150 gel lanes P7704S
NEB # PCR, Polymerases & Amplification Technologies Size Stockroom #
M0480L OneTaq® DNA Polymerase 1,000 units M0480L
M0480S OneTaq® DNA Polymerase 200 units M0480S
M0486L OneTaq® Quick-Load® 2X Master Mix with Standard Buffer 500 reactions (50 μl vol) M0486L
M0492S Q5® High-Fidelity 2X Master Mix 100 reactions (50 μl vol) M0492S
M0491S Q5® High-Fidelity DNA Polymerase 100 units M0491S
M0494S Q5® Hot Start High-Fidelity 2X Master Mix 100 rnx (2 x 1.25 ml) M0494S
M0493S Q5® Hot Start High-Fidelity DNA Polymerase 100 units M0493S
M0273L Taq DNA Polymerase with Standard Taq Buffer 2,000 units M0273L
M0273S Taq DNA Polymerase with Standard Taq Buffer 400 units M0273S
NEB # Protein Tools Size Stockroom #
P7706S Blue Prestained Protein Standard, Broad Range (11-190 kDa) 150 gel lanes P7706S
P7712S Color Prestained Protein Standard, Broad Range (11–245 kDa) 150 gel lanes P7712S
P8107S Proteinase K, Molecular Biology Grade 2 x 1 ml vials P8107S
NEB # Restriction Endonucleases Size Stockroom #
R3552S AgeI-HF® 300 units R3552S
R3136S BamHI-HF® 10,000 units R3136S
R3539S BbsI-HF® 300 units R3539S
R0144S BglII 2,000 units R0144S
R3505L EagI-HF® 2,500 units R3505L
R3101L EcoRI-HF® 50,000 units R3101L
R3101S EcoRI-HF® 10,000 units R3101S
R3104S HindIII-HF® 10,000 units R3104S
R3142S KpnI-HF® 4,000 units R3142S
R3198S MluI-HF® 1,000 units R3198S
R3193S NcoI-HF® 1,000 units R3193S
R0111S NdeI 4,000 units R0111S
R3131S NheI-HF® 1,000 units R3131S
R3189L NotI-HF® 2,500 units R3189L
R3189S NotI-HF® 500 units R3189S
R3140S PstI-HF® 10,000 units R3140S
R3156S SacI-HF® 2,000 units R3156S
R0157S SacII 2,000 units R0157S
R3122S ScaI-HF® 1,000 units R3122S
R0141S SmaI 2,000 units R0141S
R0133S SpeI 500 units R0133S
R3182S SphI-HF® 500 units R3182S
R0145S XbaI 3,000 units R0145S
R0146S XhoI 5,000 units R0146S
R0180S XmaI 500 units R0180S
NEB # RNA Reagents Size Stockroom #
N0447S Deoxynucleotide (dNTP) Solution Mix 8 μmol of each N0447S
N0446S Deoxynucleotide (dNTP) Solution Set 25 μmol of each N0446S
M0303S DNase I (RNase-free) 1,000 units M0303S
M0314S RNase Inhibitor, Murine 3,000 units M0314S
M0371S Shrimp Alkaline Phosphatase (rSAP) 500 units M0371S
M0201S T4 Polynucleotide Kinase 500 units M0201S