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University of California, Davis NEBnow®

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NEB # Buffers Size Quantity Available
B9000S BSA, Molecular Biology Grade 12 mg 2
B7204S CutSmart® Buffer 5 ml 1
B7024S Gel Loading Dye, Purple (6X) 4 ml 2
NEB # DNA Modifying Enzymes and Cloning Technologies Size Quantity Available
E2611S Gibson Assembly® Master Mix 10 reactions 4
E2621S NEBuilder® HiFi DNA Assembly Master Mix 10 reactions 3
E2621L NEBuilder® HiFi DNA Assembly Master Mix 50 reactions 1
M2200L Quick Ligation™ Kit 150 reactions 1
M2200S Quick Ligation™ Kit 30 reactions 2
M0202L T4 DNA Ligase 100,000 units 1
M0202S T4 DNA Ligase 20,000 units 5
M0202T T4 DNA Ligase 20,000 units 3
NEB # Epigenetics Size Quantity Available
R0176S DpnI 1,000 units 3
R3138S SalI-HF® 2,000 units 3
NEB # Markers & Ladders Size Quantity Available
N3232L 1 kb DNA Ladder 1 ml 1
N3232S 1 kb DNA Ladder 0.2 ml 4
N3231S 100 bp DNA Ladder 0.1 ml 4
N0468S Quick-Load® 1 kb DNA Ladder 1.25 ml 2
N0467S Quick-Load® 100 bp DNA Ladder 1.25 ml 2
N0550S Quick-Load® Purple 1 kb Plus DNA Ladder 1.25 ml 2
NEB # PCR, Polymerases & Amplification Technologies Size Quantity Available
M0210S DNA Polymerase I, Large (Klenow) Fragment 200 units 2
E3005L Luna® Universal One-Step RT-qPCR Kit 500 reactions 1
E3005S Luna® Universal One-Step RT-qPCR Kit 200 reactions 2
E3006S Luna® Universal Probe One-Step RT-qPCR Kit 200 reactions 2
E3006L Luna® Universal Probe One-Step RT-qPCR Kit 500 reactions 1
M3004S Luna® Universal Probe qPCR Master Mix 200 rxn (2 x 1 ml) 2
M3004L Luna® Universal Probe qPCR Master Mix 500 rxn (5 x 1 ml) 1
M3003L Luna® Universal qPCR Master Mix 500 rxn (5 x 1 ml) 1
M3003S Luna® Universal qPCR Master Mix 200 rxn (2 x 1 ml) 2
M0482S OneTaq® 2X Master Mix with Standard Buffer 100 reactions (50 µl vol) 2
M0480L OneTaq® DNA Polymerase 1,000 units 1
M0480S OneTaq® DNA Polymerase 200 units 2
M0530S Phusion® High-Fidelity DNA Polymerase 100 units 3
M0530L Phusion® High-Fidelity DNA Polymerase 500 units 1
M0491L Q5® High-Fidelity DNA Polymerase 500 units 2
M0491S Q5® High-Fidelity DNA Polymerase 100 units 2
M0203S T4 DNA Polymerase 150 units 2
M0273L Taq DNA Polymerase with Standard Taq Buffer 2,000 units 2
M0273S Taq DNA Polymerase with Standard Taq Buffer 400 units 2
M0267L Taq DNA Polymerase with ThermoPol® Buffer 2,000 units 2
M0267S Taq DNA Polymerase with ThermoPol® Buffer 400 units 2
NEB # Protein Tools Size Quantity Available
P7718S Blue Prestained Protein Standard, Broad Range (11-250 kDa) 150 gel lanes 2
P7719S Color Prestained Protein Standard, Broad Range (10-250 kDa) 150 gel lanes 2
P8107S Proteinase K, Molecular Biology Grade 2 x 1 ml vials 2
NEB # Restriction Endonucleases Size Quantity Available
R0117S AatII 500 units 2
R0520S AflII 2,000 units 2
R3552S AgeI-HF® 300 units 2
R0114S ApaI 5,000 units 2
R0558S AscI 500 units 2
R0174S AvrII 100 units 2
R3136S BamHI-HF® 10,000 units 2
R0144S BglII 2,000 units 2
R0175S DdeI 1,000 units 2
R3101S EcoRI-HF® 10,000 units 2
R3195S EcoRV-HF® 4,000 units 2
R3104S HindIII-HF® 10,000 units 2
R3142S KpnI-HF® 4,000 units 3
R3193S NcoI-HF® 1,000 units 3
R0111S NdeI 4,000 units 2
R3131S NheI-HF® 1,000 units 2
R3189S NotI-HF® 500 units 2
R0547S PacI 250 units 2
R0140S PstI 10,000 units 2
R3156S SacI-HF® 2,000 units 2
R0157S SacII 2,000 units 2
R0141S SmaI 2,000 units 2
R3133S SpeI-HF® 500 units 2
R3182S SphI-HF® 500 units 2
R0145S XbaI 3,000 units 3
R0146S XhoI 5,000 units 3
R0180S XmaI 500 units 3
NEB # RNA Reagents Size Quantity Available
N0447S Deoxynucleotide (dNTP) Solution Mix 8 µmol of each 2
N0446S Deoxynucleotide (dNTP) Solution Set 25 µmol of each 2
M0303S DNase I (RNase-free) 1,000 units 2
M0368L ProtoScript® II Reverse Transcriptase 10,000 units 2
M0525S Quick CIP 1,000 units 2
M0297S RNase H 250 units 2
M0307S RNase Inhibitor, Human Placenta 2,000 units 2
M0371S Shrimp Alkaline Phosphatase (rSAP) 500 units 2
M0201S T4 Polynucleotide Kinase 500 units 3

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