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University of Alabama NEBnow®

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NEB # Buffers Size Stockroom #
B9000S BSA, Molecular Biology Grade 12 mg B9000S
B7204S CutSmart® Buffer 5 ml B7204S
B7024S Gel Loading Dye, Purple (6X) 4 ml B7024S
B0202S T4 DNA Ligase Reaction Buffer 6 ml B0202S
NEB # DNA Modifying Enzymes & Cloning Technologies Size Stockroom #
E2611S Gibson Assembly® Master Mix 10 reactions E2611S
E2621S NEBuilder® HiFi DNA Assembly Master Mix 10 reactions E2621S
M0530S Phusion® High-Fidelity DNA Polymerase 100 units M0530S
E0552S Q5® Site-Directed Mutagenesis Kit (Without Competent Cells) 10 reactions E0552S
M2200S Quick Ligation™ Kit 30 reactions M2200S
M2200L Quick Ligation™ Kit 150 reactions M2200L
M0202T T4 DNA Ligase 20,000 units M0202T
M0202L T4 DNA Ligase 100,000 units M0202L
M0202S T4 DNA Ligase 20,000 units M0202S
NEB # Epigenetics Size Stockroom #
R0176S DpnI 1,000 units R0176S
R3138S SalI-HF® 2,000 units R3138S
NEB # Markers & Ladders Size Stockroom #
N3232S 1 kb DNA Ladder 0.2 ml N3232S
N3232L 1 kb DNA Ladder 1 ml N3232L
N3200L 1 kb Plus DNA Ladder 0.5 ml N3200L
N3231S 100 bp DNA Ladder 0.1 ml N3231S
N3231L 100 bp DNA Ladder 0.5 ml N3231L
N0468S Quick-Load® 1 kb DNA Ladder 1.25 ml N0468S
N0467S Quick-Load® 100 bp DNA Ladder 1.25 ml N0467S
N0552S Quick-Load® Purple 1 kb DNA Ladder 1.25 ml N0552S
N0550S Quick-Load® Purple 1 kb Plus DNA Ladder 1.25 ml N0550S
N0551S Quick-Load® Purple 100 bp DNA Ladder 1.25 ml N0551S
NEB # PCR, Polymerases & Amplification Technologies Size Stockroom #
M0210S DNA Polymerase I, Large (Klenow) Fragment 200 units M0210S
E5200S LongAmp® Taq PCR Kit 100 reactions (50 μl vol) E5200S
E3005S Luna® Universal One-Step RT-qPCR Kit 200 reactions E3005S
M3004S Luna® Universal Probe qPCR Master Mix 200 rxn (2 x 1 ml) M3004S
M3003S Luna® Universal qPCR Master Mix 200 rxn (2 x 1 ml) M3003S
M0480S OneTaq® DNA Polymerase 200 units M0480S
M0481S OneTaq® Hot Start DNA Polymerase 200 units M0481S
M0491S Q5® High-Fidelity DNA Polymerase 100 units M0491S
M0491L Q5® High-Fidelity DNA Polymerase 500 units M0491L
M0494S Q5® Hot Start High-Fidelity 2X Master Mix 100 reactions (50 μl vol) M0494S
M0493S Q5® Hot Start High-Fidelity DNA Polymerase 100 units M0493S
M0203S T4 DNA Polymerase 150 units M0203S
M0270L Taq 2X Master Mix 500 reactions (50 μl vol) M0270L
M0273S Taq DNA Polymerase with Standard Taq Buffer 400 units M0273S
M0267S Taq DNA Polymerase with ThermoPol® Buffer 400 units M0267S
M0267L Taq DNA Polymerase with ThermoPol® Buffer 2,000 units M0267L
NEB # Protein Tools Size Stockroom #
P8107S Proteinase K, Molecular Biology Grade 2 x 1 ml vials P8107S
NEB # Restriction Endonucleases Size Stockroom #
R3552S AgeI-HF® 300 units R3552S
R0558S AscI 500 units R0558S
R3136S BamHI-HF® 10,000 units R3136S
R0144S BglII 2,000 units R0144S
R3101S EcoRI-HF® 10,000 units R3101S
R3195S EcoRV-HF® 4,000 units R3195S
R3104S HindIII-HF® 10,000 units R3104S
R3142S KpnI-HF® 4,000 units R3142S
R3198S MluI-HF® 1,000 units R3198S
R3193S NcoI-HF® 1,000 units R3193S
R3131S NheI-HF® 1,000 units R3131S
R3189S NotI-HF® 500 units R3189S
R3189L NotI-HF® 2,500 units R3189L
R0547S PacI 250 units R0547S
R3156S SacI-HF® 2,000 units R3156S
R0141S SmaI 2,000 units R0141S
R0145S XbaI 3,000 units R0145S
R0146S XhoI 5,000 units R0146S
NEB # RNA Reagents Size Stockroom #
M0290S Alkaline Phosphatase, Calf Intestinal (CIP) 1,000 units M0290S
M0289S Antarctic Phosphatase 1,000 units M0289S
N0447S Deoxynucleotide (dNTP) Solution Mix 8 μmol of each N0447S
N0446S Deoxynucleotide (dNTP) Solution Set 25 μmol of each N0446S
M0303S DNase I (RNase-free) 1,000 units M0303S
E6560S ProtoScript® II First Strand cDNA Synthesis Kit 30 reactions E6560S
M0368S ProtoScript® II Reverse Transcriptase 4,000 units M0368S
M0297S RNase H 250 units M0297S
M0314S RNase Inhibitor, Murine 3,000 units M0314S
M0371S Shrimp Alkaline Phosphatase (rSAP) 500 units M0371S
M0201S T4 Polynucleotide Kinase 500 units M0201S

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