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University of Alabama NEBnow®

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NEB # Buffers Size Stockroom #
B9000S BSA, Molecular Biology Grade 12 mg B9000S
B7204S CutSmart® Buffer 5 ml B7204S
B7024S Gel Loading Dye, Purple (6X) 4 ml B7024S
B0202S T4 DNA Ligase Reaction Buffer 6 ml B0202S
NEB # DNA Modifying Enzymes & Cloning Technologies Size Stockroom #
E2611S Gibson Assembly® Master Mix 10 reactions E2611S
E2621S NEBuilder® HiFi DNA Assembly Master Mix 10 reactions E2621S
M0530S Phusion® High-Fidelity DNA Polymerase 100 units M0530S
E0552S Q5® Site-Directed Mutagenesis Kit (Without Competent Cells) 10 reactions E0552S
M2200L Quick Ligation™ Kit 150 reactions M2200L
M2200S Quick Ligation™ Kit 30 reactions M2200S
M0202T T4 DNA Ligase 20,000 units M0202T
M0202L T4 DNA Ligase 100,000 units M0202L
M0202S T4 DNA Ligase 20,000 units M0202S
NEB # Epigenetics Size Stockroom #
R0176S DpnI 1,000 units R0176S
R3138S SalI-HF® 2,000 units R3138S
NEB # Markers & Ladders Size Stockroom #
N3232S 1 kb DNA Ladder 0.2 ml N3232S
N3232L 1 kb DNA Ladder 1 ml N3232L
N3200L 1 kb Plus DNA Ladder 0.5 ml N3200L
N3231S 100 bp DNA Ladder 0.1 ml N3231S
N3231L 100 bp DNA Ladder 0.5 ml N3231L
N0468S Quick-Load® 1 kb DNA Ladder 1.25 ml N0468S
N0467S Quick-Load® 100 bp DNA Ladder 1.25 ml N0467S
N0552S Quick-Load® Purple 1 kb DNA Ladder 1.25 ml N0552S
N0550S Quick-Load® Purple 1 kb Plus DNA Ladder 1.25 ml N0550S
N0551S Quick-Load® Purple 100 bp DNA Ladder 1.25 ml N0551S
NEB # PCR, Polymerases & Amplification Technologies Size Stockroom #
M0210S DNA Polymerase I, Large (Klenow) Fragment 200 units M0210S
E5200S LongAmp® Taq PCR Kit 100 reactions (50 μl vol) E5200S
E3005S Luna® Universal One-Step RT-qPCR Kit 200 reactions E3005S
M3004S Luna® Universal Probe qPCR Master Mix 200 rxn (2 x 1 ml) M3004S
M3003S Luna® Universal qPCR Master Mix 200 rxn (2 x 1 ml) M3003S
M0480S OneTaq® DNA Polymerase 200 units M0480S
M0481S OneTaq® Hot Start DNA Polymerase 200 units M0481S
M0491S Q5® High-Fidelity DNA Polymerase 100 units M0491S
M0491L Q5® High-Fidelity DNA Polymerase 500 units M0491L
M0494S Q5® Hot Start High-Fidelity 2X Master Mix 100 reactions (50 μl vol) M0494S
M0493S Q5® Hot Start High-Fidelity DNA Polymerase 100 units M0493S
M0203S T4 DNA Polymerase 150 units M0203S
M0273S Taq DNA Polymerase with Standard Taq Buffer 400 units M0273S
M0267S Taq DNA Polymerase with ThermoPol® Buffer 400 units M0267S
M0267L Taq DNA Polymerase with ThermoPol® Buffer 2,000 units M0267L
NEB # Protein Tools Size Stockroom #
P8107S Proteinase K, Molecular Biology Grade 2 x 1 ml vials P8107S
NEB # Restriction Endonucleases Size Stockroom #
R3552S AgeI-HF® 300 units R3552S
R0558S AscI 500 units R0558S
R3136S BamHI-HF® 10,000 units R3136S
R0144S BglII 2,000 units R0144S
R3101S EcoRI-HF® 10,000 units R3101S
R3195S EcoRV-HF® 4,000 units R3195S
R3104S HindIII-HF® 10,000 units R3104S
R3142S KpnI-HF® 4,000 units R3142S
R3198S MluI-HF® 1,000 units R3198S
R3193S NcoI-HF® 1,000 units R3193S
R3131S NheI-HF® 1,000 units R3131S
R3189S NotI-HF® 500 units R3189S
R3189L NotI-HF® 2,500 units R3189L
R0547S PacI 250 units R0547S
R3156S SacI-HF® 2,000 units R3156S
R0141S SmaI 2,000 units R0141S
R0145S XbaI 3,000 units R0145S
R0146S XhoI 5,000 units R0146S
NEB # RNA Reagents Size Stockroom #
M0290S Alkaline Phosphatase, Calf Intestinal (CIP) 1,000 units M0290S
M0289S Antarctic Phosphatase 1,000 units M0289S
N0447S Deoxynucleotide (dNTP) Solution Mix 8 μmol of each N0447S
N0446S Deoxynucleotide (dNTP) Solution Set 25 μmol of each N0446S
M0303S DNase I (RNase-free) 1,000 units M0303S
E6560S ProtoScript® II First Strand cDNA Synthesis Kit 30 reactions E6560S
M0368S ProtoScript® II Reverse Transcriptase 4,000 units M0368S
M0297S RNase H 250 units M0297S
M0314S RNase Inhibitor, Murine 3,000 units M0314S
M0371S Shrimp Alkaline Phosphatase (rSAP) 500 units M0371S
M0201S T4 Polynucleotide Kinase 500 units M0201S

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