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The Jackson Laboratory NEBnow®

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NEB # Buffers Size Stockroom #
B9000S BSA, Molecular Biology Grade 12 mg B9000S
B7204S CutSmart® Buffer 5 ml B7204S
B7024S Gel Loading Dye, Purple (6X) 4 ml B7024S
NEB # DNA Modifying Enzymes and Cloning Technologies Size Stockroom #
E2611S Gibson Assembly® Master Mix 10 reactions E2611S
M0212L Klenow Fragment (3'→5' exo-) 1,000 units M0212L
M0212S Klenow Fragment (3'→5' exo-) 200 units M0212S
E6056S NEBNext® Quick Ligation Module 20 reactions E6056S
E2621S NEBuilder® HiFi DNA Assembly Master Mix 10 reactions E2621S
M2200S Quick Ligation™ Kit 30 reactions M2200S
M0202S T4 DNA Ligase 20,000 units M0202S
M0202L T4 DNA Ligase 100,000 units M0202L
M0202T T4 DNA Ligase 20,000 units M0202T
M0318L T7 DNA Ligase 750,000 units M0318L
NEB # Epigenetics Size Stockroom #
R0543M DpnII 5,000 units R0543M
M0603S EcoGII Methyltransferase 200 units M0603S
M0227L GpC Methyltransferase (M.CviPI) 1,000 units M0227L
R3138S SalI-HF® 2,000 units R3138S
NEB # Glycobiology Size Stockroom #
P0708S PNGase F, Recombinant 15,000 units P0708S
NEB # Library Preparation for Next Generation Sequencing Size Stockroom #
M6630S NEBNext® FFPE DNA Repair Mix 24 reactions M6630S
E7546S NEBNext® Ultra™ II End Repair/dA-Tailing Module 24 reactions E7546S
NEB # Markers & Ladders Size Stockroom #
N3232L 1 kb DNA Ladder 1 ml N3232L
N3232S 1 kb DNA Ladder 0.2 ml N3232S
N3231S 100 bp DNA Ladder 0.1 ml N3231S
N3231L 100 bp DNA Ladder 0.5 ml N3231L
N0551L Quick-Load® Purple 100 bp DNA Ladder 3.75 ml N0551L
NEB # Nucleic Acid Purification Size Stockroom #
T1020S Monarch® DNA Gel Extraction Kit 50 preps T1020S
T3010S Monarch® Genomic DNA Purification Kit 50 preps T3010S
T1030S Monarch® PCR & DNA Cleanup Kit (5 μg) 50 preps T1030S
T1010S Monarch® Plasmid Miniprep Kit  50 preps T1010S
NEB # PCR, Polymerases & Amplification Technologies Size Stockroom #
M0210L DNA Polymerase I, Large (Klenow) Fragment 1,000 units M0210L
M0210S DNA Polymerase I, Large (Klenow) Fragment 200 units M0210S
M0323L LongAmp® Taq DNA Polymerase 2,500 units M0323L
M0323S LongAmp® Taq DNA Polymerase 500 units M0323S
E3005S Luna® Universal One-Step RT-qPCR Kit 200 reactions E3005S
M3003S Luna® Universal qPCR Master Mix 200 rxn (2 x 1 ml) M3003S
E3010S LunaScript™ RT SuperMix Kit 25 reactions E3010S
M0541L NEBNext® High-Fidelity 2X PCR Master Mix 250 reactions M0541L
M0541S NEBNext® High-Fidelity 2X PCR Master Mix 50 reactions M0541S
M0480L OneTaq® DNA Polymerase 1,000 units M0480L
M0480S OneTaq® DNA Polymerase 200 units M0480S
M0530L Phusion® High-Fidelity DNA Polymerase 500 units M0530L
M0530S Phusion® High-Fidelity DNA Polymerase 100 units M0530S
M0531S Phusion® High-Fidelity PCR Master Mix with HF Buffer 100 reactions (50 µl vol) M0531S
M0491S Q5® High-Fidelity DNA Polymerase 100 units M0491S
M0491L Q5® High-Fidelity DNA Polymerase 500 units M0491L
M0494S Q5® Hot Start High-Fidelity 2X Master Mix 100 rxn (2 x 1.25 ml) M0494S
M0203S T4 DNA Polymerase 150 units M0203S
M0203L T4 DNA Polymerase 750 units M0203L
M0270L Taq 2X Master Mix 500 reactions (50 µl vol) M0270L
M0273S Taq DNA Polymerase with Standard Taq Buffer 400 units M0273S
M0273L Taq DNA Polymerase with Standard Taq Buffer 2,000 units M0273L
NEB # Restriction Endonucleases Size Stockroom #
R3552S AgeI-HF® 300 units R3552S
R0137L AluI 5,000 units R0137L
R0558S AscI 500 units R0558S
R3136S BamHI-HF® 10,000 units R3136S
R3733L BsaI-HF®v2 5,000 units R3733L
R3101S EcoRI-HF® 10,000 units R3101S
R3104S HindIII-HF® 10,000 units R3104S
R3142S KpnI-HF® 4,000 units R3142S
R3193S NcoI-HF® 1,000 units R3193S
R0111S NdeI 4,000 units R0111S
R3189S NotI-HF® 500 units R3189S
R3122S ScaI-HF® 1,000 units R3122S
R3133S SpeI-HF® 500 units R3133S
R0145S XbaI 3,000 units R0145S
R0146S XhoI 5,000 units R0146S
R0180S XmaI 500 units R0180S
NEB # RNA Reagents Size Stockroom #
T2010S Monarch® Total RNA Miniprep Kit 50 preps T2010S
M0289S Antarctic Phosphatase 1,000 units M0289S
N0440S dATP Solution 25 µmol N0440S
N0447S Deoxynucleotide (dNTP) Solution Mix 8 µmol of each N0447S
N0446S Deoxynucleotide (dNTP) Solution Set 25 µmol of each N0446S
T2030S Monarch® RNA Cleanup Kit (10 μg) 10 preps T2030S
T2040S Monarch® RNA Cleanup Kit (50 μg) 10 preps T2040S
T2050S Monarch® RNA Cleanup Kit (500 μg) 10 preps T2050S
M0525S Quick CIP 1,000 units M0525S
M0371S Shrimp Alkaline Phosphatase (rSAP) 500 units M0371S
M0201S T4 Polynucleotide Kinase 500 units M0201S

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