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St. Jude Children's Research Hospital NEBnow®

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Inventory as of 10/29/2020 2:41 AM

NEB # Buffers Size Quantity Available
B9000S BSA, Molecular Biology Grade 12 mg 2
B7024S Gel Loading Dye, Purple (6X) 4 ml 2
B0202S T4 DNA Ligase Reaction Buffer 6 ml 2
NEB # DNA Assembly Cloning and Mutagenesis Kits Size Quantity Available
E0552S Q5® Site-Directed Mutagenesis Kit (Without Competent Cells) 10 reactions 2
NEB # DNA Modifying Enzymes and Cloning Technologies Size Quantity Available
M0305S Endonuclease V 250 units 2
M0293L Exonuclease I (E. coli) 15,000 units 2
M0206S Exonuclease III (E. coli) 5,000 units 2
M0206L Exonuclease III (E. coli) 25,000 units 2
E2611S Gibson Assembly® Master Mix 10 reactions 2
E2611L Gibson Assembly® Master Mix 50 reactions 1
M0212S Klenow Fragment (3'→5' exo-) 200 units 2
M0212L Klenow Fragment (3'→5' exo-) 1,000 units 2
M0262L Lambda Exonuclease 5,000 units 2
E2621S NEBuilder® HiFi DNA Assembly Master Mix 10 reactions 2
E2621L NEBuilder® HiFi DNA Assembly Master Mix 50 reactions 2
M2200L Quick Ligation™ Kit 150 reactions 1
M2200S Quick Ligation™ Kit 30 reactions 2
M0202S T4 DNA Ligase 20,000 units 2
M0202L T4 DNA Ligase 100,000 units 3
M0208L Taq DNA Ligase 10,000 units 3
M5505L USER® Enzyme 250 units 3
NEB # Epigenetics Size Quantity Available
R0176S DpnI 1,000 units 2
R0543M DpnII 5,000 units 2
R0106L MspI 25,000 units 2
R3138S SalI-HF® 2,000 units 2
NEB # Genome Editing Size Quantity Available
M0386M Cas9 Nuclease, S. pyogenes 2,000 pmol 1
NEB # Markers & Ladders Size Quantity Available
N3232L 1 kb DNA Ladder 1 ml 1
N3232S 1 kb DNA Ladder 0.2 ml 2
N3231S 100 bp DNA Ladder 0.1 ml 2
N3231L 100 bp DNA Ladder 0.5 ml 1
N0552S Quick-Load® Purple 1 kb DNA Ladder 1.25 ml 2
N0551S Quick-Load® Purple 100 bp DNA Ladder 1.25 ml 2
NEB # Nucleic Acid Purification Size Quantity Available
T1020L Monarch® DNA Gel Extraction Kit 250 preps 1
T1020S Monarch® DNA Gel Extraction Kit 50 preps 1
T3010L Monarch® Genomic DNA Purification Kit 150 preps 1
T3010S Monarch® Genomic DNA Purification Kit 50 preps 1
T1030L Monarch® PCR & DNA Cleanup Kit (5 μg) 250 preps 1
T1030S Monarch® PCR & DNA Cleanup Kit (5 μg) 50 preps 1
T1010S Monarch® Plasmid Miniprep Kit  50 preps 1
T1010L Monarch® Plasmid Miniprep Kit  250 preps 1
NEB # PCR, Polymerases & Amplification Technologies Size Quantity Available
M0210S DNA Polymerase I, Large (Klenow) Fragment 200 units 2
E3005S Luna® Universal One-Step RT-qPCR Kit 200 reactions 2
M3003S Luna® Universal qPCR Master Mix 200 rxn (2 x 1 ml) 2
E3010S LunaScript™ RT SuperMix Kit 25 reactions 2
M0541L NEBNext® High-Fidelity 2X PCR Master Mix 250 reactions 1
E7645S NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® 24 reactions 1
M0544L NEBNext® Ultra™ II Q5® Master Mix 250 reactions 1
M0530L Phusion® High-Fidelity DNA Polymerase 500 units 1
M0530S Phusion® High-Fidelity DNA Polymerase 100 units 2
M0531L Phusion® High-Fidelity PCR Master Mix with HF Buffer 500 reactions (50 µl vol) 1
M0531S Phusion® High-Fidelity PCR Master Mix with HF Buffer 100 reactions (50 µl vol) 2
M0536L Phusion® Hot Start Flex 2X Master Mix 500 reactions (50 µl vol) 2
M0492S Q5® High-Fidelity 2X Master Mix 100 reactions (50 µl vol) 2
M0491S Q5® High-Fidelity DNA Polymerase 100 units 2
M0491L Q5® High-Fidelity DNA Polymerase 500 units 1
M0494L Q5® Hot Start High-Fidelity 2X Master Mix 500 rxn (10 x 1.25 ml) 1
M0494S Q5® Hot Start High-Fidelity 2X Master Mix 100 rxn (2 x 1.25 ml) 2
NEB # Protein Tools Size Quantity Available
P8107S Proteinase K, Molecular Biology Grade 2 x 1 ml vials 2
NEB # Restriction Endonucleases Size Quantity Available
R3552L AgeI-HF® 1,500 units 1
R3552S AgeI-HF® 300 units 2
R0558S AscI 500 units 2
R3136S BamHI-HF® 10,000 units 2
R0739S BsmBI-v2 200 units 2
R0739L BsmBI-v2 1,000 units 1
R3101S EcoRI-HF® 10,000 units 2
R3195S EcoRV-HF® 4,000 units 2
R3104S HindIII-HF® 10,000 units 2
R3142S KpnI-HF® 4,000 units 2
R0147M MboI 2,500 units 2
R0198S MluI 1,000 units 2
R3193S NcoI-HF® 1,000 units 2
R0111S NdeI 4,000 units 2
R3131S NheI-HF® 1,000 units 2
R0189S NotI 500 units 3
R3189S NotI-HF® 500 units 3
R3189L NotI-HF® 2,500 units 1
R0547S PacI 250 units 2
R0146S XhoI 5,000 units 2
NEB # RNA Reagents Size Quantity Available
T2010S Monarch® Total RNA Miniprep Kit 50 preps 1
N0447S Deoxynucleotide (dNTP) Solution Mix 8 µmol of each 2
M0303S DNase I (RNase-free) 1,000 units 1
E2050S HiScribe™ T7 Quick High Yield RNA Synthesis Kit 50 reactions 1
T2030S Monarch® RNA Cleanup Kit (10 μg) 10 preps 1
T2040S Monarch® RNA Cleanup Kit (50 μg) 10 preps 1
T2050S Monarch® RNA Cleanup Kit (500 μg) 10 preps 1
M0525S Quick CIP 1,000 units 2
M0297S RNase H 250 units 1
M0314S RNase Inhibitor, Murine 3,000 units 1
M0314L RNase Inhibitor, Murine 15,000 units 1
M0201S T4 Polynucleotide Kinase 500 units 2
M0201L T4 Polynucleotide Kinase 2,500 units 3

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