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Roswell Park Cancer Institute

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NEB # Buffers Size Stockroom #
B9000S BSA, Molecular Biology Grade 12 mg B9000S
B7204S CutSmart® Buffer 5 ml B7204S
B7024S Gel Loading Dye, Purple (6X) 4 ml B7024S
B7021S Gel Loading Dye, Blue (6X) 4 ml B7021S
NEB # DNA Modifying Enzymes & Cloning Technologies Size Stockroom #
E2621L NEBuilder® HiFi DNA Assembly Master Mix 50 reactions E2621L
M0530S Phusion® High-Fidelity DNA Polymerase 100 units M0530S
M0530L Phusion® High-Fidelity DNA Polymerase 500 units M0530L
M2200S Quick Ligation™ Kit 30 reactions M2200S
M0202S T4 DNA Ligase 20,000 units M0202S
M0202L T4 DNA Ligase 100,000 units M0202L
NEB # Epigenetics Size Stockroom #
R0176S DpnI 1,000 units R0176S
R3138S SalI-HF® 2,000 units R3138S
NEB # Markers & Ladders Size Stockroom #
N3232S 1 kb DNA Ladder 0.2 ml N3232S
N3231L 100 bp DNA Ladder 0.5 ml N3231L
N3239S Quick-Load® 1 kb Extend DNA Ladder 1.25 ml N3239S
N0467S Quick-Load® 100 bp DNA Ladder 1.25 ml N0467S
N0550S Quick-Load® Purple 2-Log DNA Ladder (0.1 - 10.0 kb) 1.25 ml N0550S
NEB # PCR, Polymerases & Amplification Technologies Size Stockroom #
M0210S DNA Polymerase I, Large (Klenow) Fragment 200 units M0210S
E3005L Luna® Universal One-Step RT-qPCR Kit 500 reactions E3005L
E3005S Luna® Universal One-Step RT-qPCR Kit 200 reactions E3005S
E3006S Luna® Universal Probe One-Step RT-qPCR Kit 200 reactions E3006S
M3004S Luna® Universal Probe qPCR Master Mix 200 rxn (2 x 1 ml) M3004S
M3003L Luna® Universal qPCR Master Mix 500 rxn (5 x 1 ml) M3003L
M3003S Luna® Universal qPCR Master Mix 200 rxn (2 x 1 ml) M3003S
M0484L OneTaq® Hot Start 2X Master Mix with Standard Buffer 500 reactions (50 μl vol) M0484L
M0488L OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer 500 reactions (50 μl vol) M0488L
M0486L OneTaq® Quick-Load® 2X Master Mix with Standard Buffer 500 reactions (50 μl vol) M0486L
M0531L Phusion® High-Fidelity PCR Master Mix with HF Buffer 500 reactions (50 μl vol) M0531L
M0531S Phusion® High-Fidelity PCR Master Mix with HF Buffer 100 reactions (50 μl vol) M0531S
M0491S Q5® High-Fidelity DNA Polymerase 100 units M0491S
M0491L Q5® High-Fidelity DNA Polymerase 500 units M0491L
M0494S Q5® Hot Start High-Fidelity 2X Master Mix 100 reactions (50 μl vol) M0494S
M0494L Q5® Hot Start High-Fidelity 2X Master Mix 500 reactions (50 μl vol) M0494L
M0493S Q5® Hot Start High-Fidelity DNA Polymerase 100 units M0493S
M0203S T4 DNA Polymerase 150 units M0203S
M0267S Taq DNA Polymerase with ThermoPol® Buffer 400 units M0267S
M0267L Taq DNA Polymerase with ThermoPol® Buffer 2,000 units M0267L
NEB # Protein Tools Size Stockroom #
P7706S Blue Prestained Protein Standard, Broad Range (11-190 kDa) 150 gel lanes P7706S
P7712S Color Prestained Protein Standard, Broad Range (11–245 kDa) 150 gel lanes P7712S
NEB # Restriction Endonucleases Size Stockroom #
R0114S ApaI 5,000 units R0114S
R3136S BamHI-HF® 10,000 units R3136S
R0144S BglII 2,000 units R0144S
R0197S ClaI 1,000 units R0197S
R3101S EcoRI-HF® 10,000 units R3101S
R3195S EcoRV-HF® 4,000 units R3195S
R3104S HindIII-HF® 10,000 units R3104S
R0105S HpaI 500 units R0105S
R3142S KpnI-HF® 4,000 units R3142S
R3193S NcoI-HF® 1,000 units R3193S
R0111S NdeI 4,000 units R0111S
R3131S NheI-HF® 1,000 units R3131S
R3189S NotI-HF® 500 units R3189S
R0547S PacI 250 units R0547S
R3140S PstI-HF® 10,000 units R3140S
R3156S SacI-HF® 2,000 units R3156S
R3122S ScaI-HF® 1,000 units R3122S
R0141S SmaI 2,000 units R0141S
R3133S SpeI-HF® 500 units R3133S
R0145S XbaI 3,000 units R0145S
R0146S XhoI 5,000 units R0146S
NEB # RNA Reagents Size Stockroom #
M0290S Alkaline Phosphatase, Calf Intestinal (CIP) 1,000 units M0290S
N0447S Deoxynucleotide (dNTP) Solution Mix 8 μmol of each N0447S
N0446S Deoxynucleotide (dNTP) Solution Set 25 μmol of each N0446S
M0253L M-MuLV Reverse Transcriptase 50,000 units M0253L
E6300S ProtoScript® First Strand cDNA Synthesis Kit 30 reactions E6300S
E6560S ProtoScript® II First Strand cDNA Synthesis Kit 30 reactions E6560S
M0368S ProtoScript® II Reverse Transcriptase 4,000 units M0368S
M0314S RNase Inhibitor, Murine 3,000 units M0314S
M0371S Shrimp Alkaline Phosphatase (rSAP) 500 units M0371S
M0201S T4 Polynucleotide Kinase 500 units M0201S

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