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Pagliuca Harvard Life Lab NEBnow

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Inventory as of 10/31/2020 2:41 AM

B9000S BSA, Molecular Biology Grade 12 mg 2
B7204S CutSmart® Buffer 5 ml 2
NEB # DNA Assembly Cloning and Mutagenesis Kits Size Quantity Available
E0552S Q5® Site-Directed Mutagenesis Kit (Without Competent Cells) 10 reactions 1
NEB # DNA Modifying Enzymes and Cloning Technologies Size Quantity Available
M0367L Blunt/TA Ligase Master Mix 250 reactions 2
E2611S Gibson Assembly® Master Mix 10 reactions 2
E6056L NEBNext® Quick Ligation Module 100 reactions 3
E2621S NEBuilder® HiFi DNA Assembly Master Mix 10 reactions 2
M2200S Quick Ligation™ Kit 30 reactions 2
M0202S T4 DNA Ligase 20,000 units 2
M0202L T4 DNA Ligase 100,000 units 1
M0302L T7 Endonuclease I 1,250 units 3
M5505L USER® Enzyme 250 units 2
NEB # Epigenetics Size Quantity Available
R0176S DpnI 1,000 units 2
NEB # Genome Editing Size Quantity Available
M0386M Cas9 Nuclease, S. pyogenes 2,000 pmol 1
NEB # Markers & Ladders Size Quantity Available
N3232S 1 kb DNA Ladder 0.2 ml 2
N3200S 1 kb Plus DNA Ladder 0.1 ml 2
N3231S 100 bp DNA Ladder 0.1 ml 2
N0552S Quick-Load® Purple 1 kb DNA Ladder 1.25 ml 2
N0550S Quick-Load® Purple 1 kb Plus DNA Ladder 1.25 ml 2
N0551S Quick-Load® Purple 100 bp DNA Ladder 1.25 ml 2
NEB # NGS Sample Prep & Target Enrichment Size Quantity Available
M6630L NEBNext® FFPE DNA Repair Mix 96 reactions 3
E7546L NEBNext® Ultra™ II End Repair/dA-Tailing Module 96 reactions 2
NEB # Nucleic Acid Purification Size Quantity Available
T1020S Monarch® DNA Gel Extraction Kit 50 preps 1
T1030S Monarch® PCR & DNA Cleanup Kit (5 μg) 50 preps 1
T1010S Monarch® Plasmid Miniprep Kit  50 preps 1
NEB # PCR, Polymerases & Amplification Technologies Size Quantity Available
M0287L LongAmp® Taq 2X Master Mix 500 reactions (50 µl vol) 2
E3005S Luna® Universal One-Step RT-qPCR Kit 200 reactions 2
E3006S Luna® Universal Probe One-Step RT-qPCR Kit 200 reactions 2
M3003S Luna® Universal qPCR Master Mix 200 rxn (2 x 1 ml) 2
M0480S OneTaq® DNA Polymerase 200 units 2
M0530S Phusion® High-Fidelity DNA Polymerase 100 units 2
M0531S Phusion® High-Fidelity PCR Master Mix with HF Buffer 100 reactions (50 µl vol) 2
M0491S Q5® High-Fidelity DNA Polymerase 100 units 2
M0494S Q5® Hot Start High-Fidelity 2X Master Mix 100 rxn (2 x 1.25 ml) 2
M0203S T4 DNA Polymerase 150 units 1
NEB # Protein Tools Size Quantity Available
P8107S Proteinase K, Molecular Biology Grade 2 x 1 ml vials 4
NEB # Restriction Endonucleases Size Quantity Available
R0652S AfeI 200 units 2
R3552S AgeI-HF® 300 units 2
R0174S AvrII 100 units 1
R3539S BbsI-HF® 300 units 2
R3101S EcoRI-HF® 10,000 units 2
R3104S HindIII-HF® 10,000 units 2
R3189S NotI-HF® 500 units 2
R3127S NsiI-HF® 1,000 units 1
R3122S ScaI-HF® 1,000 units 1
R3133S SpeI-HF® 500 units 2
R3182S SphI-HF® 500 units 2
R0145S XbaI 3,000 units 2
R0146S XhoI 5,000 units 2
R0180S XmaI 500 units 2
NEB # RNA Reagents Size Quantity Available
M0289S Antarctic Phosphatase 1,000 units 2
N0447S Deoxynucleotide (dNTP) Solution Mix 8 µmol of each 2
N0446S Deoxynucleotide (dNTP) Solution Set 25 µmol of each 2
M0303S DNase I (RNase-free) 1,000 units 2
E6560S ProtoScript® II First Strand cDNA Synthesis Kit 30 reactions 2
M0314S RNase Inhibitor, Murine 3,000 units 1
M0371S Shrimp Alkaline Phosphatase (rSAP) 500 units 1
M0201S T4 Polynucleotide Kinase 500 units 2

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