Harvard Medical School Biopolymers Facility


  • Harvard Medical School
    Biopolymers Facilities Self Service Supply Center
    088 New Research Building, 77 Avenue Louis Pasteur
    Boston, MA 02115
    Visit this freezer's website
    https://genome.med.harvard.edu
    Hours

    Monday - Friday, 9:00 am - 5:00 pm

    Contact
    James Caffery
    Phone (617) 432-7480
    Fax (617) 432-7440
    Your NEB Sales Rep
    Joanna Hamilton

    Phone 978-471-2387

Available in this freezer

NEB # Buffers Size Stockroom #
B7024S Gel Loading Dye, Purple (6X) 4 ml B7024S
B7021S Gel Loading Dye, Blue (6X) 4 ml B7021S
T1031L Monarch® DNA Cleanup Binding Buffer 235 ml T1031L
T1016L Monarch® DNA Elution Buffer 25 ml T1016L
T1032L Monarch® DNA Wash Buffer 25 ml T1032L
T1021L Monarch® Gel Dissolving Buffer 235 ml T1021L
T1012L Monarch® Plasmid Lysis Buffer (B2) 2 x 27 ml T1012L
T1013L Monarch® Plasmid Neutralization Buffer (B3) 110 ml T1013L
T1014L Monarch® Plasmid Wash Buffer 1 2 x 27 ml T1014L
T1015L Monarch® Plasmid Wash Buffer 2 30 ml T1015L
NEB # Competent Cells Size Stockroom #
C2987H NEB® 5-alpha Competent E. coli (High Efficiency) 20 x 0.05 ml/tube C2987H
C2989K NEB® 5-alpha Electrocompetent E. coli 6 x 0.1 ml/tube C2989K
C2992H NEB® 5-alpha F'Iq Competent E. coli (High Efficiency) 20 x 0.05 ml/tube C2992H
NEB # DNA Modifying Enzymes and Cloning Technologies Size Stockroom #
M0293L Exonuclease I (E. coli) 15,000 units M0293L
E2611S Gibson Assembly® Master Mix 10 reactions E2611S
E2611L Gibson Assembly® Master Mix 50 reactions E2611L
M0212S Klenow Fragment (3'→5' exo-) 200 units M0212S
M0212L Klenow Fragment (3'→5' exo-) 1,000 units M0212L
E2612S NEBNext® Microbiome DNA Enrichment Kit 6 reactions E2612S
E2621S NEBuilder® HiFi DNA Assembly Master Mix 10 reactions E2621S
E2621L NEBuilder® HiFi DNA Assembly Master Mix 50 reactions E2621L
M2200L Quick Ligation™ Kit 150 reactions M2200L
M2200S Quick Ligation™ Kit 30 reactions M2200S
M0202S T4 DNA Ligase 20,000 units M0202S
M0202L T4 DNA Ligase 100,000 units M0202L
M0202M T4 DNA Ligase 100,000 units M0202M
M0208S Taq DNA Ligase 2,000 units M0208S
NEB # Epigenetics Size Stockroom #
R0176L DpnI 5,000 units R0176L
R0176S DpnI 1,000 units R0176S
R3138S SalI-HF® 2,000 units R3138S
NEB # Markers & Ladders Size Stockroom #
N3232L 1 kb DNA Ladder 1 ml N3232L
N3232S 1 kb DNA Ladder 0.2 ml N3232S
N3200S 1 kb Plus DNA Ladder 0.1 ml N3200S
N3231S 100 bp DNA Ladder 0.1 ml N3231S
N3231L 100 bp DNA Ladder 0.5 ml N3231L
N3233S Low Molecular Weight DNA Ladder 0.1 ml N3233S
NEB # Nucleic Acid Purification Size Stockroom #
T1034L Monarch® DNA Cleanup Columns (5 μg) 100 columns T1034L
T1020S Monarch® DNA Gel Extraction Kit 50 preps T1020S
T1020L Monarch® DNA Gel Extraction Kit 250 preps T1020L
T1030S Monarch® PCR & DNA Cleanup Kit (5 μg) 50 preps T1030S
T1030L Monarch® PCR & DNA Cleanup Kit (5 μg) 250 preps T1030L
T1017L Monarch® Plasmid Miniprep Columns 100 columns T1017L
T1010L Monarch® Plasmid Miniprep Kit  250 preps T1010L
T1010S Monarch® Plasmid Miniprep Kit  50 preps T1010S
NEB # PCR, Polymerases & Amplification Technologies Size Stockroom #
M0210S DNA Polymerase I, Large (Klenow) Fragment 200 units M0210S
M0287S LongAmp® Taq 2X Master Mix 100 reactions (50 µl vol) M0287S
M0532S Phusion® High-Fidelity PCR Master Mix with GC Buffer 100 reactions (50 µl vol) M0532S
M0530S Phusion® High-Fidelity DNA Polymerase 100 units M0530S
M0530L Phusion® High-Fidelity DNA Polymerase 500 units M0530L
M0531S Phusion® High-Fidelity PCR Master Mix with HF Buffer 100 reactions (50 µl vol) M0531S
M0492L Q5® High-Fidelity 2X Master Mix 500 reactions (50 µl vol) M0492L
M0492S Q5® High-Fidelity 2X Master Mix 100 reactions (50 µl vol) M0492S
M0491L Q5® High-Fidelity DNA Polymerase 500 units M0491L
M0491S Q5® High-Fidelity DNA Polymerase 100 units M0491S
M0203L T4 DNA Polymerase 750 units M0203L
M0273L Taq DNA Polymerase with Standard Taq Buffer 2,000 units M0273L
M0273S Taq DNA Polymerase with Standard Taq Buffer 400 units M0273S
NEB # Protein Tools Size Stockroom #
P8107S Proteinase K, Molecular Biology Grade 2 x 1 ml vials P8107S
NEB # Restriction Endonucleases Size Stockroom #
R3552S AgeI-HF® 300 units R3552S
R3136S BamHI-HF® 10,000 units R3136S
R3539L BbsI-HF® 1,500 units R3539L
R3539S BbsI-HF® 300 units R3539S
R0144S BglII 2,000 units R0144S
R0531S BsaAI 500 units R0531S
R3101S EcoRI-HF® 10,000 units R3101S
R3195S EcoRV-HF® 4,000 units R3195S
R3104S HindIII-HF® 10,000 units R3104S
R0589S MfeI 500 units R0589S
R3589S MfeI-HF® 500 units R3589S
R3198S MluI-HF® 1,000 units R3198S
R3193S NcoI-HF® 1,000 units R3193S
R0111S NdeI 4,000 units R0111S
R3131S NheI-HF® 1,000 units R3131S
R3189L NotI-HF® 2,500 units R3189L
R3189S NotI-HF® 500 units R3189S
R0133S SpeI 500 units R0133S
R3182S SphI-HF® 500 units R3182S
R0145S XbaI 3,000 units R0145S
R0146S XhoI 5,000 units R0146S
NEB # RNA Reagents Size Stockroom #
M0289S Antarctic Phosphatase 1,000 units M0289S
T2040S Monarch® RNA Cleanup Kit (50 μg) 10 preps T2040S
T2050S Monarch® RNA Cleanup Kit (500 μg) 10 preps T2050S
M0201S T4 Polynucleotide Kinase 500 units M0201S
M0201L T4 Polynucleotide Kinase 2,500 units M0201L