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HomeFAQsCan whole blood be used as template for cDNA synthesis?
FAQ: Can whole blood be used as template for cDNA synthesis?
Whole blood can successfully be used as template for cDNA synthesis if additional magnesium is supplemented to the reaction. We recommend increasing the final MgCl2 (NEB #B9021) concentration by 3 mM at 1X in the cDNA synthesis reaction.
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