Yes. Most PCR buffers are compatible. No additional reaction buffer is necessary if the enzyme is added to a PCR reaction.
Incubation in the following buffers at 55°C for 15 minutes resulted in the relative activity listed:
|r1.1 (NEB# B7030)||20|
|r2.1 (NEB# B6002)||50|
|r3.1 (NEB# B6003)||50|
|rCutSmartTM (NEB# B6004)||100|
|Exonuclease I Reaction Buffer (NEB# B0293)||10|
|ThermoPol (NEB# B9004)||90|