HomeFAQsIs Faustovirus Capping Enzyme sensitive to secondary structure?
FAQ: Is Faustovirus Capping Enzyme sensitive to secondary structure?
Yes. Secondary structures that limit 5′ end accessibility will reduce capping yield. Incubating capping reactions at higher temperatures or pre-heating the sample prior to adding capping enzyme can help improve capping of structured substrates. In severe cases, altering the sequence to the transcript to reduce secondary structure may be required.